Development of a polyvinyl alcohol/sodium alginate hydrogel-based scaffold incorporating bFGF-encapsulated microspheres for accelerated wound healing
Development of a polyvinyl alcohol/sodium alginate hydrogel-based scaffold incorporating bFGF-encapsulated microspheres for accelerated wound healing
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DOI：
10.1038/s41598-020-64480-9
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发表时间：
2020-04-30
影响因子：
4.6
通讯作者：
Nokoorani, Yeganeh Dorri
中科院分区：
文献类型：
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作者：
Bahadoran, Maedeh;Shamloo, Amir;Nokoorani, Yeganeh Dorri
在本研究中，一个混合微球/水凝胶系统，由聚乙烯醇（PVA）/海藻酸钠（SA）水凝胶纳入PCL微球作为皮肤支架，以加速伤口愈合。使用冻融方法开发水凝胶基质，并基于体外评估优化其结构中所涉及的聚合物的比例。利用双乳化溶剂蒸发技术制备了bFGF包裹的PCL微球。然后通过体外和体内实验对所获得的冻干杂交系统进行表征。结果表明，随着SA浓度的增加，水凝胶的孔结构增多，溶胀能力、弹性和降解速率提高，但最大强度和断裂伸长率降低。PCL微球嵌入到优化的水凝胶结构中提供了bFGF的持续和无爆发释放动力学。此外，载药微球的加入导致水凝胶基质的降解机制没有显着变化，但它降低了其机械强度。此外，MTT试验表明杂交系统没有细胞毒性作用。在烧伤创面大鼠模型上的体内研究，包括伤口闭合机制的评价和组织学分析表明，所制造的支架有效地促进了细胞诱导的组织再生和烧伤创面愈合。
In the present study, a hybrid microsphere/hydrogel system, consisting of polyvinyl alcohol (PVA)/sodium alginate (SA) hydrogel incorporating PCL microspheres is introduced as a skin scaffold to accelerate wound healing. The hydrogel substrate was developed using the freeze-thawing method, and the proportion of the involved polymers in its structure was optimized based on the in-vitro assessments. The bFGF-encapsulated PCL microspheres were also fabricated utilizing the double-emulsion solvent evaporation technique. The achieved freeze-dried hybrid system was then characterized by in-vitro and in-vivo experiments. The results obtained from the optimization of the hydrogel showed that increasing the concentration of SA resulted in a more porous structure, and higher swelling ability, elasticity and degradation rate, but decreased the maximum strength and elongation at break. The embedding of PCL microspheres into the optimized hydrogel structure provided sustained and burst-free release kinetics of bFGF. Besides, the addition of drug-loaded microspheres led to no significant change in the degradation mechanism of the hydrogel substrate; however, it reduced its mechanical strength. Furthermore, the MTT assay represented no cytotoxic effect for the hybrid system. The in-vivo studies on a burn-wound rat model, including the evaluation of the wound closure mechanism, and histological analyses indicated that the fabricated scaffold efficiently contributed to promoting cell-induced tissue regeneration and burn-wound healing.