HIGH MITOCHONDRIAL DIVERSITY OF APIS MELLIFERA UNDER COI GENE FROM CHINA AND PAKISTAN
HIGH MITOCHONDRIAL DIVERSITY OF APIS MELLIFERA UNDER COI GENE FROM CHINA AND PAKISTAN
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中国和巴基斯坦 COI 基因下 APIS mellifera 线粒体高度多样性
DOI：
10.15666/aeer/1603_29332945
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发表时间：
2018-01-01
影响因子：
0.7
通讯作者：
Su, S.
中科院分区：
文献类型：
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作者：
Rizwan, M.;Li, Z.;Su, S.
利用细胞色素氧化酶亚基（COI）基因对中国和巴基斯坦10个不同地点的240只蜜蜂进行了遗传多样性分析。筛选序列后，利用BioEdit软件进行比对，比对后的序列应用于MEGA-7软件，通过Neighbor-Joining法与所有可用亚种进行系统发育关系分析。另一方面，利用MEGA7通过Kimura-2-Parameter计算遗传散度，利用DnaSP v5计算遗传多样性。系统进化树结果将所有居群划分为2个类群中的7个亚种，总体遗传差异最大（3.88%），巴基斯坦居群次之（2.65%），中国居群最小（2.02%）。总体遗传多样性显著，共有23个突变，Fu’s Fu概率值为0.019。通过Fu的Fu和D测试，国家观察显示巴基斯坦具有非常显著的变异性，以及17个突变，而中国表现出较少的多样性，只有10个突变。此外，核苷酸错配分布的计算也证实了我们所有测序数据之间的遗传变异性。因此，我们的研究结果揭示了来自两个不同国家的蜜蜂亚种之间强烈的遗传相互作用和显著的遗传变异。这些结果对研究不同生态区蜜蜂亚种间的亲缘关系具有重要意义。
The genetic diversity of honeybee populations (240 individual) Apis mellifera from ten various locations of China and Pakistan was investigated by using COI (Cytochrome Oxidase Subunit I) gene. After screening of sequences, BioEdit was used for the alignment, and these aligned sequences were employed to MEGA-7 software, to find out the phylogenetic relationship with all available subspecies through Neighbor-Joining method. On the other hand, genetic divergence was calculated via Kimura-2-Parameter using MEGA7, whereas genetic diversity was calculated by DnaSP v5. Phylogenetic tree results divided all populations into seven subspecies within two groups and maximum genetic divergence was observed overall at 3.88%, followed by Pakistan at 2.65% while minimum divergence value (2.02%) was observed for China Likewise, significant genetic diversity overall was observed with 23 mutations, confirmed with the probability value of Fu's Fu at 0.019. Country-wise observations revealed that Pakistan had much highly significant variability through Fu's Fu and D tests, along with 17 mutations whereas China had shown less diversity with ten mutations. Moreover, the calculation of nucleotide mismatch distribution had also confirmed genetic variability among all our sequenced data. Thus, our findings have revealed the strong genetic interaction and a significant genetic variation among all seven subspecies of A. mellifera from two different countries. These results could be more helpful to investigate the genetic relationship among other subspecies of A. mellifera from different ecological zones.