Hyperspectral imaging fluorescence excitation scanning spectral characteristics of remodeled mouse arteries.
Hyperspectral imaging fluorescence excitation scanning spectral characteristics of remodeled mouse arteries.
复制标题
重塑小鼠动脉的高光谱成像荧光激发扫描光谱特征。
DOI：
10.1117/12.2510770
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Leavesley,SilasJ
中科院分区：
文献类型：
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作者：
Deal,Joshua;McFarland,StuartJ;Robinson,Anna;Alford,Anna;Weber,DavidS;Rich,ThomasC;Leavesley,SilasJ
冠状动脉疾病（CAD），或动脉粥样硬化，是造成近三分之一的美国人每年死亡。斑块的检测和斑块阶段的分化仍然是治疗的复杂因素。在严重堵塞或破裂之前对斑块进行分类可以为临床决策提供信息并预防死亡。目前的检测方法要么是非特异性的，缓慢的，或需要使用潜在有害的造影剂。高光谱成像的最新进展可用于检测与血管重塑和随后的斑块形成相关的动脉自发荧光的变化，并可检测和分类现有病变。在这里，我们提出的数据比较光谱图像特征的小鼠模型设计进行血管重塑。C57 Bl/6小鼠进行结扎的三个尾侧分支的左颈总动脉（左颈外动脉，颈内动脉，枕动脉）与上级甲状腺动脉根据IACUC批准的协议保持完整。在不同的时间点采集血管，以比较重塑程度，包括术后4周和5个月。在收获后立即通过纵向打开以将管腔表面暴露于20 X物镜来制备血管。将具有微弧灯和薄膜可调谐滤光器阵列（Versachrome，Semrock，Inc.）用于实现光谱成像。激发扫描使用340 nm和550 nm之间的波长，增量为5 nm。高光谱数据的生成和分析与自定义Matlab脚本和可视化ENVI。初步数据表明与对照和重塑血管相关的一致光谱特征。
Coronary artery disease (CAD), or atherosclerosis, is responsible for nearly a third of all American deaths annually. Detection of plaques and differentiation of plaque stage remains a complicating factor for treatment. Classification of plaque before significant blockage or rupture could inform clinical decisions and prevent mortality. Current detection methods are either nonspecific, slow, or require the use of potentially harmful contrast agents. Recent advances in hyperspectral imaging could be used to detect changes in the autofluorescence of arteries associated with vessel remodeling and subsequent plaque formation and could detect and classify existing lesions. Here, we present data comparing spectral image characteristics of a mouse model designed to undergo vessel remodeling.C57Bl/6 mice underwent ligation of three of four caudal branches of the left common carotid artery (left external carotid, internal carotid, and occipital artery) with the superior thyroid artery left intact under IACUC approved protocol. Vessels were harvested at a variety of timepoints to compare degrees of remodeling, including 4 weeks and 5 months post-surgery. Immediately following harvest, vessels were prepared by longitudinal opening to expose the luminal surface to a 20X objective. A custom inverted microscope (TE-2000, Nikon Instruments) with a Xe arc lamp and thin film tunable filter arrary (Versachrome, Semrock, Inc.) were used to achieve spectral imaging. Excitation scans utilized wavelengths between 340 nm and 550 nm in 5 nm increments. Hyperspectral data were generated and analyzed with custom Matlab scripts and visualized in ENVI. Preliminary data suggest consistent spectral features associated with control and remodeled vessels.