Lysozyme adsorption onto mesoporous materials: effect of pore geometry and stability of adsorbents.
Lysozyme adsorption onto mesoporous materials: effect of pore geometry and stability of adsorbents.
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DOI：
10.1166/jnn.2007.511
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发表时间：
2007-03
影响因子：
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通讯作者：
A. Vinu;Masahiko Miyahara;K. Hossain;Motoi Takahashi;V. Balasubramanian;T. Mori;K. Ariga
中科院分区：
文献类型：
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作者：
A. Vinu;Masahiko Miyahara;K. Hossain;Motoi Takahashi;V. Balasubramanian;T. Mori;K. Ariga
本文研究了两种介孔吸附剂（KIT-5和AISBA-15）对溶菌酶的吸附，并讨论了孔结构和吸附剂稳定性对吸附效果的影响。KIT-5介孔二氧化硅材料具有笼型孔几何形状，而AISBA-15吸附剂具有直径相当大（9.7 nm）的圆柱型介孔。溶菌酶在AISBA-15铝硅酸盐上的吸附遵循Langmuir等温线，导致25至30%的孔占据。相比之下，KIT-5吸附剂对溶菌酶吸附的吸附容量非常小，通常为孔占据的6 - 13%。笼型KIT-5吸附剂具有窄通道（4至6 nm），其中溶菌酶（3 x 3 x 4.5 nm）的渗透可能受到限制。KIT-5吸附剂在长时间浸泡在水中后趋于崩溃，如XRD图案所示，而AISBA-15吸附剂即使在碱性水中浸泡4天后仍保持其规则结构。这些事实证实了与KIT-5介孔硅酸盐相比，AISBA-15作为吸附剂的优越性。研究结果表明，介孔材料的选择是实现生物材料在水环境中高效固定化的关键。
In this paper, adsorption of lysozyme onto two kinds of mesoporous adsorbents (KIT-5 and AISBA-15) has been investigated and the results on the effects of pore geometry and stability of the adsorbents are also discussed. The KIT-5 mesoporous silica materials possess cage-type pore geometry while the AISBA-15 adsorbent has mesopores of cylindrical type with rather large diameter (9.7 nm). Adsorption of lysozyme onto AISBA-15 aluminosilicate obeys a Langmuir isotherm, resulting in pore occupation of 25 to 30%. In contrast, the KIT-5 adsorbents showed very small adsorption capacities for the lysozyme adsorption, typically falling in 6 to 13% of pore occupation. The cage-type KIT-5 adsorbents have narrow channel (4 to 6 nm) where penetration of the lysozyme (3 x 3 x 4.5 nm) might be restricted. The KIT-5 adsorbent tends to collapse after long-time immersion in water, as indicated by XRD patterns, while the AISBA-15 adsorbent retains its regular structure even after immersion in basic water for 4 days. These facts confirm superiority of the AISBA-15 as an adsorbent as compared with the KIT-5 mesoporous silicates. This research strikingly demonstrates the selection of mesoporous materials is crucial to achieve efficient immobilization of biomaterials in aqueous environment.