BCL2 antisense transcripts decrease intracellular Bcl2 expression and sensitize LNCaP prostate cancer cells to apoptosis-inducing agents.
BCL2 antisense transcripts decrease intracellular Bcl2 expression and sensitize LNCaP prostate cancer cells to apoptosis-inducing agents.
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BCL2 反义转录物可降低细胞内 Bcl2 表达并使 LNCaP 前列腺癌细胞对凋亡诱导剂敏感。
DOI：
10.1089/108497801753354320
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
deVereWhite,RW
中科院分区：
文献类型：
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作者：
Shi,XB;Gumerlock,PH;Muenzer,JT;deVereWhite,RW
前列腺癌（CaP）是老年男性最常见的癌症，也是美国男性癌症死亡的第二大原因。由于Bcl2被认为具有保护CaP细胞免于凋亡的作用，我们研究了下调Bcl2表达对CaP细胞的影响。用表达BCL2反义（BCL2- as）转录的质粒稳定转染LNCaP细胞，建立基因工程LNCaP亚系。Western blotting分析显示，转染Bcl2 - as的LNCaP细胞胞内Bcl2蛋白减少50-60%。反义转录物的表达导致LNCaP细胞在雄激素戒断后生长抑制50%，并使这些细胞对阿霉素诱导的凋亡显着敏感。这些结果表明，利用Bcl2 - as转录物下调Bcl2蛋白可用于改善晚期CaP的治疗。
Prostate cancer (CaP) is the most commonly diagnosed cancer of aging men and the second leading cause of male cancer death in the United States. At present, no effective therapy is available for treating hormone independent CaP. Since Bcl2 is believed to play a role in protecting CaP cells from apoptosis, we investigated the effects of down-regulating Bcl2 expression on CaP cells. Genetically engineered LNCaP sublines were established by stably transfecting LNCaP cells with BCL2 antisense (BCL2-AS) transcript-expressing plasmids. Western blotting analysis showed that intracellular Bcl2 protein was decreased by 50–60% in BCL2-AS-transfected LNCaP cells. Expression of the antisense transcripts resulted in 50% growth inhibition of LNCaP cells in response to androgen withdrawal and markedly sensitized these cells to Adriamycin-induced apoptosis. These results suggest that down-regulation of Bcl2 protein using BCL2-AS transcripts could be exploited for improved treatment of advanced CaP.