Xylose-Insensitive Direct Electron Transfer Biosensor Strip with Single-Walled Carbon Nanotubes and Novel Fungal Flavin Adenine Dinucleotide Glucose Dehydrogenase
Xylose-Insensitive Direct Electron Transfer Biosensor Strip with Single-Walled Carbon Nanotubes and Novel Fungal Flavin Adenine Dinucleotide Glucose Dehydrogenase
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具有单壁碳纳米管和新型真菌黄素腺嘌呤二核苷酸葡萄糖脱氢酶的木糖不敏感直接电子转移生物传感器条
DOI：
10.1109/jsen.2020.3001606
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发表时间：
2020
影响因子：
4.3
通讯作者：
H. Muguruma,
中科院分区：
文献类型：
--
作者：
H. Iwasa;A. Hiratsuka;T. Tanaka;K. Tsuji;T. Kishimoto;Y. Watanabe;Y. Hoshino;H. Muguruma,
用于糖尿病自身血糖监测的商用生物传感器条是介导型电子转移(MET)，其中使用电子转移介体。本文首次研制了木糖不敏感的直接电子转移(DET)葡萄糖生物传感器条。它克服了MET同行的缺点。用胆酸钠(SC)表面活性剂脱束的单壁碳纳米管(SWCNTs)位于黄素腺嘌呤二核苷酸葡萄糖脱氢酶(FAD-GDH)反应中心口袋附近的DET构筑。与此同时，研究发现，百里香酚(TM)与不使用时相比，DET电流增加了7倍。TM没有特定的氧化还原电位，没有碳纳米管(CNTs)也不起作用，但在CNTs和FAD-GDH之间辅助DET。因此，它不是电子转移介体，而是DET增效剂。长期储存测试表明，与MET生物传感器电极不同，GDH/TM/SWCNT-SC电极不会自然还原。由富含糖链的新型真菌FAD-GDH、SWCNTs、Sc和Tm在水溶液中组成的“生物墨水”适合于制作对木糖不敏感的DET生物传感器条。所制得的DET生物传感器条所需进样量为900nL(1.5×0.1×6.0 mm)，测量范围为0-600 mg/mL，测量时间为10 S，具有重复测量能力，实际样品测量的变异系数为5%。低成本的生物传感器将得到广泛的推广，从而为实现健康社会做出贡献。
Commercial biosensor strip for self-blood sugar monitoring for diabetes is type of mediated electron transfer (MET), where an electron transfer mediator is used. Here, a xylose-insensitive direct electron transfer (DET) glucose biosensor strip is developed for the first time. It overcomes the disadvantages of MET counterparts. A DET formation in which single-walled carbon nanotubes (SWCNTs), debundled using sodium cholate (SC) surfactant, are positioned near the reaction center pocket of flavin adenine dinucleotide glucose dehydrogenase (FAD-GDH) is constructed. Concomitantly, it is found that thymol (TM) increases the DET current seven times compared to when it is not used. TM does not have a specific redox potential and does not work without carbon nanotubes (CNTs), but assisted DET between the CNTs and FAD-GDH. Therefore, it is not an electron transfer mediator but a DET enhancement chemical. Long-term storage tests show that GDH/TM/SWCNT-SC electrodes are not naturally reduced unlike MET biosensor electrodes. The “bionanoink,” composed of glycan chain rich novel fungal FAD-GDH, SWCNTs, SC, and TM in aqueous solution is suitable for the fabrication of a DET biosensor strip that is insensitive to xylose. The resulting DET biosensor strip had the required sample volume of 900 nL (1.5 × 0.1 × 6.0 mm), a measurement range of 0-600 mg/mL, measurement time of 10 s, the ability for repeated measurements, and a 5% coefficient of variation in real sample measurements. A low cost biosensor will be widely spread, consequently, will contribute to the realization of a healthy society.