Development of a split SNAP-tag protein complementation assay for visualization of protein-protein interactions in living cells
Development of a split SNAP-tag protein complementation assay for visualization of protein-protein interactions in living cells
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DOI：
10.1039/c2an35762c
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Kobatake, Eiry
中科院分区：
文献类型：
--
作者：
Mie, Masayasu;Naoki, Tatsuhiko;Kobatake, Eiry
建立了一种分离SNAP-Tag蛋白质互补分析方法，用于显示活细胞中蛋白质-蛋白质的相互作用。分离的SNAP-TAG是在第91和92个氨基酸残基之间分离的SNAP-Tag片段，与能够相互作用的蛋白质融合。在与荧光SNAP-Tag底物孵育后，表达分裂的SNAP-Tag融合蛋白的细胞在这些蛋白相互作用时产生荧光信号。此外，通过与拆分片段标签(SNAP-Tag突变体)相结合，实现了同时标记。这种分离的SNAP-Tag标记方法对于蛋白质-蛋白质相互作用过程的可视化是一个有用的工具。
A split SNAP-tag protein complementation assay was developed for visualization of protein-protein interactions in living cells. Split SNAP-tagms, fragments of divided SNAP-tag between amino acid residues 91 and 92, were fused to proteins that can interact with each other. After incubation with a fluorescent SNAP-tag substrate, cells that expressed split SNAP-tag fusion proteins generated fluorescent signals when these proteins interacted. Moreover, by combination with the split CLIP-tag (SNAP-tag mutant), simultaneous labeling was achieved. This split SNAP-tag labeling method should be a useful tool for visualization of protein-protein interaction processes.