TRKB, A NEURAL RECEPTOR PROTEIN-TYROSINE KINASE - EVIDENCE FOR A FULL-LENGTH AND 2 TRUNCATED RECEPTORS
TRKB, A NEURAL RECEPTOR PROTEIN-TYROSINE KINASE - EVIDENCE FOR A FULL-LENGTH AND 2 TRUNCATED RECEPTORS
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DOI：
10.1128/mcb.11.1.143
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发表时间：
1991-01-01
影响因子：
5.3
通讯作者：
HUNTER, T
中科院分区：
文献类型：
--
作者：
MIDDLEMAS, DS;LINDBERG, RA;HUNTER, T
我们筛选了一个成年大鼠小脑cDNA文库，以寻找新的蛋白酪氨酸激酶(PTK)cDNAs。克隆了一个可能的PTK基因TrkB，其序列表明它可能来源于与人类trk癌基因密切相关的配体调节受体的基因。Northern(RNA)分析表明，TrkB基因主要在脑内表达，TrkB表达多种mRNAs，大小从0.7 kb到9kb不等。大脑mRNAs与多种探针的杂交表明，存在编码TrkB受体截短的mRNAs。另外两种类型的基因被分离出来，它们的序列被预测为编码两种截短的受体，这两种受体具有完整的胞外区和跨膜区，但在短的细胞质尾巴上不同。
We have screened an adult rat cerebellar cDNA library in search of novel protein tyrosine-kinase (PTK) cDNAs. A cDNA for a putative PTK, trkB, was cloned, and its sequence indicates that it is likely to be derived from a gene for a ligand-regulated receptor closely related to the human trk oncogene. Northern (RNA) analysis showed that the trkB gene is expressed predominantly in the brain and that trkB expresses multiple mRNAs, ranging from 0.7 to 9 kb. Hybridization of cerebral mRNAs with a variety of probes indicates that there are mRNAs encoding truncated trkB receptors. Two additional types of cDNA were isolated, and their sequences are predicted to encode two distinct C-terminally truncated receptors which have the complete extracellular region and transmembrane domain, but which differ in their short cytoplasmic tails.