Studies on oxidation and reduction by pneumococcus. II. The production of peroxide by sterile extracts of pneumococcus
Studies on oxidation and reduction by pneumococcus. II. The production of peroxide by sterile extracts of pneumococcus
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DOI：
10.1084/jem.39.3.357
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发表时间：
1924-03-01
影响因子：
15.3
通讯作者：
Neil, JM
中科院分区：
文献类型：
--
作者：
Avery, OT;Neil, JM
1.未洗涤的肺炎球菌的无菌肉汤提取物，完全不含活细胞或完整细胞，可有效还原亚甲基蓝。2.通过在磷酸盐溶液中提取洗涤的肺炎球菌制备的无菌提取物本身不能还原亚甲蓝。在加入肉浸液或酵母提取物后，这些洗涤过的细胞提取物积极地减少亚甲基蓝。3.肺炎球菌提取物中负责亚甲基蓝还原的一个或多个系统通过暴露于与先前发现的破坏相同提取物的过氧化物形成活性的温度几乎相同的温度而被破坏。4.有人建议，过氧化物的形成和亚甲蓝还原肺炎球菌提取物是相同或密切相关的系统的功能，诱导的特定反应取决于分子氧或亚甲蓝是否作为氢受体或氧供体。
1. Sterile broth extracts of unwashed pneumococci, entirely free from living or intact cells, actively reduce methylene blue. 2. Sterile extracts prepared by extracting washed pneumococci in phosphate solution are unable by themselves to reduce methylene blue. Upon the addition of meat infusion or yeast extract, these washed cell extracts actively reduce methylene blue. 3. The system or systems in pneumococcus extracts responsible for methylene blue reduction are destroyed by exposure to temperatures practically identical with those which have been previously found to destroy the peroxide-forming activity of the same extracts. 4. It is suggested that peroxide formation and methylene blue reduction by pneumococcus extracts are functions of the same or closely related systems, the particular reaction induced depending upon whether molecular oxygen or methylene blue serves as hydrogen acceptor or oxygen donator.