The development of photochemically crosslinked native fibrinogen as a rapidly formed and mechanically strong surgical tissue sealant
The development of photochemically crosslinked native fibrinogen as a rapidly formed and mechanically strong surgical tissue sealant
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DOI：
10.1016/j.biomaterials.2008.12.059
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发表时间：
2009-04-01
期刊：
影响因子：
14
通讯作者：
Werkmeister, Jerome A.
中科院分区：
文献类型：
--
作者：
Elvin, Christopher M.;Brownlee, Alan G.;Werkmeister, Jerome A.
我们最近报道了通过使用可见光照明的快速光化学过程产生高弹性的交联蛋白质生物材料。根据这些发现，我们预测其他未修饰的、富含酪氨酸的自缔合蛋白质也可能对这种共价交联方法敏感。在这里，我们表明，未修饰的天然纤维蛋白原也可以通过快速形成分子间二酪氨酸光化学交联成弹性水凝胶生物材料。光化学交联纤维蛋白原形成的组织封闭剂粘合强度至少是商业纤维蛋白胶的5倍，并且能够在20 s内产生最大粘合强度。体外研究表明，光化学交联反应的组分对细胞无毒。该材料将在需要快速固化以实现高强度组织密封的各种外科手术中找到有用的应用。皇冠版权所有（C）2009由爱思唯尔有限公司出版。保留所有权利。
We recently reported the generation of a highly elastic, crosslinked protein biomaterial via a rapid photochemical process using visible light illumination. In light of these findings, we predicted that other unmodified, tyrosine-rich, self-associating proteins might also be susceptible to this covalent crosslinking method. Here we show that unmodified native fibrinogen can also be photochemically crosslinked into an elastic hydrogel biomaterial through the rapid formation of intermolecular dityrosine. Photochemically crosslinked fibrinogen forms tissue sealant bonds at least 5-fold stronger than commercial fibrin glue and is capable of producing maximum bond strength within 20 s. In vitro studies showed that components of the photochemical crosslinking reaction are non-toxic to cells. This material will find useful application in various surgical procedures where rapid curing for high strength tissue sealing is required. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.