Simultaneous determination of psychoactive drugs and their metabolites in aqueous matrices by liquid chromatography mass spectrometry
Simultaneous determination of psychoactive drugs and their metabolites in aqueous matrices by liquid chromatography mass spectrometry
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DOI：
10.1021/es061740w
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发表时间：
2006-12-01
影响因子：
11.4
通讯作者：
Ternes, Thomas A.
中科院分区：
文献类型：
--
作者：
Hummel, Daniela;Loeffler, Dirk;Ternes, Thomas A.
开发了一种多残留方法，可以同时测定原液和处理过的废水、地表水、地下水和饮用水中的精神活性化合物，如阿片类药物、镇定剂、抗癫痫药（primidone、卡马西平加两种代谢物）、可卡因代谢物苯甲酰ecgonine、抗抑郁药多虑平以及钙通道阻滞剂维拉帕米。在中性pH条件下，Oasis HLB固相萃取后，采用正离子模式的LC电喷雾串联质谱法检测。除少数例外，分析物的相对回收率超过70%。定量限在低ng/L范围内。使用适当的氘化或c -13- n -15标记的替代标准来补偿基质效应。对于原液和处理后的废水，降低浓度因子以降低基质效应。大多数分析物（20种中的15种）存在于原始和处理过的废水以及地表水中，因此，可能在环境中无处不在。抗癫痫药、鸦片生物碱吗啡和可待因、二氢可待因、两种镇定剂恶西泮和替马西泮、阿片类药物曲马多、多塞平和维拉帕米在STP排放和德国河流中的浓度达到Ag/L范围。在饮用水中，只有卡马西平及其代谢物10,11-二羟基-10,11-二氢卡马西平和primidone的浓度高达0.020 Ag/L。
A multi-residue method was developed that allows for the simultaneous determination of psychoactive compounds such as opioids, tranquilizers, antiepileptics (primidone, carbamazepine plus two metabolites), the cocaine metabolite benzoylecgonine, the antidepressant doxepin, as well as the calcium channel blocker verapamil in raw and treated wastewater, surface water, groundwater, and drinking water. After solid-phase extraction with Oasis HLB at neutral pH, the analytes were detected by LC electrospray tandem MS in the positive ion mode. With a few exceptions relative recoveries of the analytes exceeded 70%. The limits of quantification were in the low ng/L range. Matrix effects were compensated by using appropriate deuterated or C-13-N-15-labeled surrogate standards. For raw and treated wastewater, concentration factors were lowered to reduce matrix effects. Most analytes (15 of 20) were found in raw and treated wastewater as well as in surface water, and hence, are presumably ubiquitously present in the environment. Antiepileptics, the opium alkaloids morphine and codeine, dihydrocodeine, the two tranquilizers oxazepam and temazepam, the opioid tramadol, doxepin, and verapamil were detected in STP discharges and German rivers at concentrations up to the Ag/L range. In drinking water, only carbamazepine, its metabolite 10,11-dihydroxy-10,11- dihydrocarbamazepine, and primidone were present at concentrations up to 0.020 Ag/L.