Is the expression of sense and antisense transgenes really sufficient for artificial piRNA production?
Is the expression of sense and antisense transgenes really sufficient for artificial piRNA production?
复制标题
有义和反义转基因的表达真的足以用于人工 piRNA 的生产吗？
DOI：
10.1016/j.cub.2015.07.001
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发表时间：
2015
期刊：
影响因子：
9.2
通讯作者：
Keisuke Shoji and Susumu Katsuma
中科院分区：
文献类型：
--
作者：
Izumi N;Shoji K;Sakaguchi Y;Honda S;Kirino Y;Suzuki T;Katsuma S;Tomari Y;Keisuke Shoji and Susumu Katsuma
动物已经进化出一套优雅的防御系统来对抗各种各样的自私因子，比如转座子。在动物生殖系细胞中，PIWI蛋白和与PIWI蛋白相关的小rna （pirna）是这一防御系统的核心。pirna是23 - 30nt长的小rna，作为PIWI蛋白的序列特异性向导。PIWI蛋白具有由pirna引导的切片器活性；因此PIWI-piRNA复合物通过切割转座子rna[1]来沉默转座子活性。目前，novopiRNA是如何针对一个新的非自体元件产生的，这在很大程度上是未知的。Itouet al.[2]最近使用报告基因转基因小鼠进行的一项研究得出结论，正义和反义RNA转录物的同时表达足以产生piRNA。然而，我们使用相同的piRNA数据集进行的生物信息学分析表明，仅引入反义报告基因结构就能产生转基因衍生的piRNA，这与Itouet al. bb0的部分结论不一致。
Animals have evolved an elegant defense system against a diverse range of selfish elements such as transposons. In animal germ line cells, PIWI proteins and small RNAs associated with PIWI proteins (piRNAs) are at the heart of this defense system. piRNAs are 23–30-nt-long small RNAs that act as sequence-specific guides for PIWI proteins. PIWI proteins possess a slicer activity that is guided by piRNAs; the PIWI–piRNA complex thus silences transposon activity by cleaving transposon RNAs [1]. At present, howde novopiRNA production occurs against a new non-self element is largely unknown. A recent study by Itouet al.[2] using reporter transgenic mice concluded that the concomitant expression of sense and antisense RNA transcripts is sufficient for piRNA production. Our bioinformatic analysis using the same piRNA datasets, however, demonstrates that the introduction of the antisense reporter construct alone produces transgene-derived piRNAs, which is inconsistent with a part of the conclusions of Itouet al.[2].