Genetic variability and evolutionary diversification of membrane ABC transporters in plants.
Genetic variability and evolutionary diversification of membrane ABC transporters in plants.
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DOI：
10.1186/s12870-014-0323-2
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发表时间：
2015-02-13
影响因子：
5.3
通讯作者：
Ercolano MR
中科院分区：
文献类型：
--
作者：
Andolfo G;Ruocco M;Di Donato A;Frusciante L;Lorito M;Scala F;Ercolano MR
ATP结合盒蛋白被认为在所有生命领域的生长和抗性过程的调节中起着至关重要的作用。由于它们在耐药性中的作用，它们在脊椎动物中已被深入研究，但对ABC超家族在植物中的功能知之甚少。最近公布的植物基因组序列使我们能够在四种维管植物（水稻）中鉴定出803种ABC转运蛋白。sativa、Solanum lycopersicum、Solanum tuberosum和葡萄属vinifera）和绿色团藻（Volvox carteri）中的76个转运蛋白，并与拟南芥（Arabidopsis thaliana）和酿酒酵母（Saccharomyces cerevisiae）中的转运蛋白进行比较。回收的蛋白质进行了遗传学分析，以推断同源关系。在A、D、E和F亚家族中发现了大多数的直系亲属关系，并观察和讨论了ABCG亚家族中有趣的扩展。在ABC的五个亚家族A、B、C、D和E中进行了高水平的净化选择。然而，最近的重复基因的进化速率可能会影响维管植物基因组的多样性。番茄器官内ABC基因的转录谱揭示了一些转运蛋白的广泛功能作用和其他转运蛋白的更特异的活性，表明番茄中存在关键的ABC调节因子。在这项工作中取得的发现可能有助于解决有关不同物种基因组之间关系的进化的几个生物学问题。所获得的植物ABC蛋白质库存可能是基础和应用研究的有价值的工具。事实上，插入基因功能的假定作用可以加速发现新的ABC超家族成员。本文的在线版本（doi：10.1186/s12870-014-0323-2）包含补充材料，可供授权用户使用。
ATP-binding cassette proteins have been recognized as playing a crucial role in the regulation of growth and resistance processes in all kingdoms of life. They have been deeply studied in vertebrates because of their role in drug resistance, but much less is known about ABC superfamily functions in plants. Recently released plant genome sequences allowed us to identify 803 ABC transporters in four vascular plants (Oryza. sativa, Solanum lycopersicum, Solanum tuberosum and Vitis vinifera) and 76 transporters in the green alga Volvox carteri, by comparing them with those reannotated in Arabidopsis thaliana and the yeast Saccharomyces cerevisiae. Retrieved proteins have been phylogenetically analysed to infer orthologous relationships. Most orthologous relationships in the A, D, E and F subfamilies were found, and interesting expansions within the ABCG subfamily were observed and discussed. A high level of purifying selection is acting in the five ABC subfamilies A, B, C, D and E. However, evolutionary rates of recent duplicate genes could influence vascular plant genome diversification. The transcription profiles of ABC genes within tomato organs revealed a broad functional role for some transporters and a more specific activity for others, suggesting the presence of key ABC regulators in tomato. The findings achieved in this work could contribute to address several biological questions concerning the evolution of the relationship between genomes of different species. Plant ABC protein inventories obtained could be a valuable tool both for basic and applied studies. Indeed, interpolation of the putative role of gene functions can accelerate the discovering of new ABC superfamily members. The online version of this article (doi:10.1186/s12870-014-0323-2) contains supplementary material, which is available to authorized users.