Activation of Met tyrosine kinase by hepatocyte growth factor is essential for internal organogenesis in Xenopus embryo.
Activation of Met tyrosine kinase by hepatocyte growth factor is essential for internal organogenesis in Xenopus embryo.
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肝细胞生长因子激活 Met 酪氨酸激酶对于非洲爪蟾胚胎的内部器官发生至关重要。
DOI：
10.1006/bbrc.1997.6567
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发表时间：
1997
影响因子：
3.1
通讯作者：
Toshikazu Nakamura
中科院分区：
文献类型：
--
作者：
S. Aoki;Kuniaki Takahashi;Kunio Matsumoto;Toshikazu Nakamura
肝细胞生长因子 (HGF) 特异性激活 Met 酪氨酸激酶受体，导致多种细胞发生有丝分裂、运动发生和形态发生反应。为了了解 HGF 在非洲爪蟾胚胎发生中的作用，通过截短的酪氨酸激酶阴性 Met 的显性表达引入了功能丧失突变。当酪氨酸激酶阴性 Met mRNA 被微注射到两细胞至八细胞阶段的爪蟾胚胎中时，肝脏发育大部分受到损害，前肾和肠道的结构在受限的发育后期严重发育不全。这些结果强烈表明，HGF 和 Met 之间的功能耦合对于源自原始肠道并可能参与胚胎骨骼发生的内脏器官的发育至关重要。连同 HGF 或 Met 基因突变小鼠的发育异常，HGF 对肝脏发育的重要作用从两栖动物到哺乳动物物种都高度保守。
Hepatocyte growth factor (HGF) specifically activates Met tyrosine kinase receptor, leading to mitogenic, motogenic, and morphogenic responses in a wide variety of cells. To know a role of HGF in Xenopus embryogenesis, loss-of-function mutation was introduced by dominant expression of truncated tyrosine kinase-negative Met. When tyrosine kinase-negative Met mRNA was micro-injected into two-cell to eight-cell stages Xenopus embryos, the liver development was mostly impaired and structures of pronephros and the gut were grossly underdeveloped in the restricted, late stage of development. These results strongly suggest that functional coupling between HGF and Met is essential for the development of internal organs originated from primitive gut and possibly involved in embryonic skeletogenesis. Together with developmental abnormality in mice mutated with HGF or Met gene, essential role of HGF for liver development is highly conserved from amphibian to mammalian species.
影响因子：
10.5
作者：
Gualdi, R;Bossard, P;Zaret, KS
通讯作者：
Zaret, KS