Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation
Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation
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DOI：
10.1007/s00253-007-0951-7
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发表时间：
2007-07-01
影响因子：
5
通讯作者：
Chen, Zhiyi
中科院分区：
文献类型：
--
作者：
Li, Dequan;Nie, Fengya;Chen, Zhiyi
枯草芽孢杆菌916是防治水稻纹枯病的有效生防菌。为了进一步提高其拮抗能力，对Bs-916进行了低能离子注入。我们研究了不同剂量的N~+注入的效应。Bs-916的最佳离子注入剂量为15 × 2.6 × 10（14）N +/cm（2）至25 × 2.6 × 10（14）N +/cm（2）。通过平板筛选，获得了一株具有较高抑菌活性的突变株Bs-H74。其对指示菌的抑菌圈比亲本菌株增加了30.7%。对水稻纹枯病的防效比Bs-916提高14.6%。薄层层析和高效液相色谱分析表明，Bs-916和突变株产生的脂肽属于表面活性素家族。与Bs-916相比，Bs-H74产生约3.0倍的表面活性素。为了确定表面活性素在Bs-916生防中的作用，我们测试了另一株表面活性素水平显著降低的突变株Bs-M49，发现Bs-M49对R.索拉尼这些结果表明，Bs-916产生的表面活性素在抑制纹枯病中起重要作用。这些观察结果还表明，Bs-H74突变菌株是比亲本菌株更好的生防剂。
Bacillus subtilis 916 was an effective biocontrol agent in control rice sheath blight caused by Rhizoctonia solani. To further improve its antagonistic ability, low-energy ion implantation was applied in Bs-916. We studied the effects of different doses of N+ implantation. The optimum dose of ion implantation for the Bs-916 was from 15 x 2.6 x 10(14) N+ cm(2) to 25 x 2.6 x 10(14) N+/cm(2). The mutant strain designated as Bs-H74 was obtained, which showed higher inhibition activity in the screening plate. Its inhibition zone against the indicator organism increased by 30.7% compared to the parental strain. The control effect of rice sheath blight was improved by 14.6% over that of Bs-916. Thin-layer chromatography and high-performance liquid chromatography analysis indicated that lipopeptides produced by Bs-916 and the mutant strains belonged to the surfactin family. Bs-H74 produced approximately 3.0-fold surfactin compared to that of Bs-916. To determine the role of surfactin in biocontrol by Bs-916, we tested another mutant strain, Bs-M49, which produced lower levels of surfactin significantly, and found that Bs-M49 had no obvious effects against R. solani. These results suggested that the surfactin produced by Bs-916 plays an important role in the suppression of sheath blight. These observations also showed that the Bs-H74 mutant strain is a better biocontrol agent than the parental strain.