Phytosterol glycosides reduce cholesterol absorption in humans
Phytosterol glycosides reduce cholesterol absorption in humans
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DOI：
10.1152/ajpgi.00001.2009
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发表时间：
2009-04-01
影响因子：
4.5
通讯作者：
Ostlund, Richard E., Jr.
中科院分区：
文献类型：
--
作者：
Lin, Xiaobo;Ma, Lina;Ostlund, Richard E., Jr.
膳食植物甾醇抑制肠道胆固醇吸收，调节全身胆固醇排泄和平衡。然而，它们在生物化学上是异质的，并且在某些食物中有一部分被糖基化，对生物活性的影响未知。我们测试了植物甾醇糖苷减少人体胆固醇吸收的假设。采用两步法从大豆卵磷脂中提取纯化植物甾醇苷。胆固醇吸收测定在一系列的三个单餐测试，每隔2周给予11名健康受试者。在一项随机交叉设计中，参与者接受了类似于300 mg植物甾醇糖苷或植物甾醇酯形式的植物甾醇，或安慰剂，测试早餐也含有30 mg胆固醇-d（7）。每次试验后4-5天，通过血浆胆固醇-d（7）富集的质谱法估计胆固醇吸收。与安慰剂试验相比，植物甾醇糖苷降低胆固醇吸收37.6 +/- 4.8%（P < 0.0001），植物甾醇酯降低30.6 +/- 3.9%（P = 0.0001）。这些结果表明，从卵磷脂中纯化的天然植物甾醇糖苷在人体内具有生物活性，应包括在植物甾醇分析方法和食品植物甾醇含量表中。
Dietary phytosterols inhibit intestinal cholesterol absorption and regulate whole body cholesterol excretion and balance. However, they are biochemically heterogeneous and a portion is glycosylated in some foods with unknown effects on biological activity. We tested the hypothesis that phytosterol glycosides reduce cholesterol absorption in humans. Phytosterol glycosides were extracted and purified from soy lecithin in a novel two-step process. Cholesterol absorption was measured in a series of three single-meal tests given at intervals of 2 wk to each of 11 healthy subjects. In a randomized crossover design, participants received similar to 300 mg of added phytosterols in the form of phytosterol glycosides or phytosterol esters, or placebo in a test breakfast also containing 30 mg cholesterol-d(7). Cholesterol absorption was estimated by mass spectrometry of plasma cholesterol-d(7) enrichment 4-5 days after each test. Compared with the placebo test, phytosterol glycosides reduced cholesterol absorption by 37.6 +/- 4.8% (P < 0.0001) and phytosterol esters 30.6 +/- 3.9% (P = 0.0001). These results suggest that natural phytosterol glycosides purified from lecithin are bioactive in humans and should be included in methods of phytosterol analysis and tables of food phytosterol content.