Transcriptomic changes in Nicotiana benthamiana plants inoculated with the wild-type or an attenuated mutant of Tobacco vein banding mosaic virus
Transcriptomic changes in Nicotiana benthamiana plants inoculated with the wild-type or an attenuated mutant of Tobacco vein banding mosaic virus
复制标题
接种烟草脉带花叶病毒野生型或减毒突变体的本塞姆氏烟草植物的转录组变化
DOI：
10.1111/mpp.12471
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发表时间：
2017-10-01
影响因子：
4.9
通讯作者：
Li, Xiang-Dong
中科院分区：
文献类型：
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作者：
Geng, Chao;Wang, Hong-Yan;Li, Xiang-Dong
烟草叶脉带花叶病毒（TVBMV）是一种主要侵染茄类作物的多聚病毒。多型病毒的辅助成分蛋白酶（HCpro）是一种RNA沉默抑制蛋白，决定不同多型病毒（包括TVBMV）引起的疾病症状的严重程度。已有研究表明，在TVBMV感染克隆的HCpro开放阅读框（ORF）中引入替代突变，导致Asp（189）变为Lys或Ile(250)-Gln（251）变为Asp-Glu （Asp，天冬氨酸；Gln，谷氨酰胺；Glu，谷氨酸；Ile，异亮氨酸）。这些氨基酸的变化消除了突变体HCpro （HCm）的RNA沉默抑制活性，减轻了突变体TVBMV （T-HCm）在本烟植物中引起的疾病症状。本研究采用rna测序技术比较了接种野生型TVBMV （T-WT）的植株与接种T-HCm的植株在农渗后1、2和10天的基因表达情况。在接种T-WT或T-HCm的叶片中，1和2 dpai时，与翻译机制相关的benthamiana基因上调，而与脂质生物合成和代谢或细胞外/外部刺激反应相关的基因下调。在10 dpai时，T-WT感染抑制了光合作用相关基因。T-WT和T-HCm感染不同程度地干扰了参与RNA沉默途径的基因。T-WT感染后，水杨酸和乙烯信号通路受到诱导，茉莉酸信号通路受到抑制。T-WT和T-HCm的感染也会对生长素信号转导相关基因进行不同的调控，这与TVBMV引起的发育迟缓表型有关。这些结果在转录组学水平上说明了TVBMV感染在benthamiana中的动态性质。
Tobacco vein banding mosaic virus (TVBMV) is a potyvirus which mainly infects solanaceous crops. The helper component proteinase (HCpro) of a potyvirus is an RNA silencing suppressor protein and determines the severity of disease symptoms caused by different potyviruses, including TVBMV. It has been shown that substitution mutations introduced into the HCpro open reading frame (ORF) in a TVBMV infectious clone result in changes of Asp(189) to Lys or Ile(250)-Gln(251) to Asp-Glu (Asp, aspartic acid; Gln, glutamine; Glu, glutamic acid; Ile, isoleucine). These amino acid changes eliminate the RNA silencing suppression activity of the mutant HCpro (HCm) and attenuate the disease symptoms caused by the mutant TVBMV (T-HCm) in Nicotiana benthamiana plants. Here, we used RNA-sequencing technology to compare gene expression in plants inoculated with the wild-type TVBMV (T-WT) with that in plants inoculated with T-HCm at 1, 2 and 10 days post-agroinfiltration (dpai). At 1 and 2 dpai, N. benthamiana genes related to the translation machinery were up-regulated, whereas genes related to lipid biosynthesis and metabolism or to responses to extracellular/external stimuli were down-regulated in leaves inoculated with T-WT or T-HCm. At 10 dpai, T-WT infection repressed photosynthesis-related genes. T-WT and T-HCm infections differentially perturbed the genes involved in the RNA silencing pathway. The salicylic acid and ethylene signalling pathways were induced, but the jasmonic acid signalling pathway was repressed after T-WT infection. Infections of T-WT and T-HCm also differentially regulated the genes involved in auxin signalling transduction, which is known to associate with the stunting phenotypes caused by TVBMV. These results illustrate the dynamic nature of TVBMV infection in N. benthamiana at the transcriptomic level.