THE PH IN THE PRECORNEAL TEAR FILM AND UNDER A CONTACT-LENS MEASURED WITH A FLUORESCENT-PROBE
THE PH IN THE PRECORNEAL TEAR FILM AND UNDER A CONTACT-LENS MEASURED WITH A FLUORESCENT-PROBE
复制标题
DOI：
10.1016/0014-4835(90)90209-d
复制
发表时间：
1990-03-01
影响因子：
3.4
通讯作者：
MAURICE, DM
中科院分区：
文献类型：
--
作者：
CHEN, FS;MAURICE, DM
通过滴注pH敏感的荧光染料吡喃，非侵入性地确定人眼角膜前泪膜的反应。平均值为7.83（S.D.）.+-. 0.10），并且在眼睑保持关闭后睁开眼睛时立即采用该值。HCO3系统似乎只负责泪膜的一部分缓冲能力。当滴入用0.075 M PO4缓冲至pH 6.4的液滴时，泪液在约7 min内恢复至其正常值，与结膜囊中溶质的洗脱时间一致。在透气或不透气的隐形眼镜后面的泪液中建立了7.3的pH值，这可能是由于它们限制了眼睛中CO2的损失。兔角膜前泪膜碱性更强，pH值约为8.2。
The reaction of the precorneal tear film of the human eye was determined non-invasively by instilling pyranine, a pH-sensitive fluorescent dye. The mean value was 7.83 (S.D. .+-. 0.10) and it takes up this value immediately on opening the eye after the lids had been kept closed. The HCO3 system seems to be responsible for only a portion of the buffering power of the tear film. When a drop buffered to pH 6.4 with 0.075 M PO4 was instilled, the tears returned to their normal value in about 7 min, consistent with the washout time of solutes in the conjunctival sac. A pH of 7.3 was established in the tear fluid behind contact lenses, either gas permeable or impermeable, probably as a result of their restricting the loss of CO2 from the eye. The rabbit pre-corneal tear film is more alkaline, at about pH 8.2.