ROUS-SARCOMA VIRUS-TRANSFORMED FIBROBLASTS AND CELLS OF MONOCYTIC ORIGIN DISPLAY A PECULIAR DOT-LIKE ORGANIZATION OF CYTOSKELETAL PROTEINS INVOLVED IN MICROFILAMENT MEMBRANE INTERACTIONS
ROUS-SARCOMA VIRUS-TRANSFORMED FIBROBLASTS AND CELLS OF MONOCYTIC ORIGIN DISPLAY A PECULIAR DOT-LIKE ORGANIZATION OF CYTOSKELETAL PROTEINS INVOLVED IN MICROFILAMENT MEMBRANE INTERACTIONS
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DOI：
10.1016/0014-4827(87)90238-2
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发表时间：
1987-03-01
影响因子：
3.7
通讯作者：
TARONE, G
中科院分区：
文献类型：
--
作者：
MARCHISIO, PC;CIRILLO, D;TARONE, G
通过免疫荧光和干涉反射显微镜（IRM），我们发现F - 肌动蛋白以及一组参与微丝 - 膜相互作用的细胞骨架蛋白，包括纽蛋白、α - 辅肌动蛋白、丝束蛋白和踝蛋白，在单核细胞以及单核细胞衍生的细胞（如巨噬细胞和破骨细胞）中，特异性地组织成离散的点状结构，这些结构与细胞 - 基质接触位点相对应。这些蛋白具有精确的拓扑分布；纽蛋白和踝蛋白形成一个类似甜甜圈的环，而肌动蛋白、丝束蛋白和α - 辅肌动蛋白则组织成与环相匹配的点状。在劳斯肉瘤病毒（RSV）转化的恶性成纤维细胞中也检测到F - 肌动蛋白及相关细胞骨架蛋白相同的点状组织形式，但在培养的相应未转化细胞中未检测到。由此得出结论，RSV转化诱导成纤维细胞表达一种细胞骨架组织和一种黏附模式，这种模式通常在单核细胞起源的细胞中发现。我们提出，这种细胞骨架组织在RSV转化的成纤维细胞和单核细胞衍生细胞中的出现可能反映了一种跨越解剖边界迁移的共同能力。
By immunofluorescence and interference reflection microscopy (IRM) we found that F-actin and a group of cytoskeletal proteins involved in microfilament-membrane interaction, including vinculin, .alpha.-actinin, fimbrin and talin, are specifically organized in discrete dot-like structures corresponding to cell-substratum contact sites in both monocytes and monocyte-derived cells such as macrophages and osteoclasts. These proteins have a precise topological distribution; vinculin and talin form a doughnut-like ring, while actin, fimbrin and .alpha.-actinin are organized in dots matching the rings. An identical dot-like organization of F-actin and associated cytoskeletal proteins was also detected in malignant fibroblasts transformed by Rous Sarcoma virus (RSV) but not in the corresponding untransformed cells in culture. It is concluded that RSV transformation induces fibroblasts to express a cytoskeletal organization and a pattern of adhesion that are normally found in cells of monocytic origin. We propose that the occurrence of this cytoskeletal organization in RSV-transformed fibroblasts and in monocyte-derived cells may reflect a common ability to migrate across anatomical boundaries.