The effect of probiotic treatment with Clostridium butyricum on enterohemorrhagic Escherichia coli O157:H7 infection in mice
The effect of probiotic treatment with Clostridium butyricum on enterohemorrhagic Escherichia coli O157:H7 infection in mice
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DOI：
10.1016/j.femsim.2004.03.010
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发表时间：
2004-07-01
影响因子：
--
通讯作者：
Kamiya, S
中科院分区：
文献类型：
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作者：
Takahashi, M;Taguchi, H;Kamiya, S
肠出血性大肠杆菌（EHEC） 0 157:117被认为是导致出血性结肠炎和溶血性尿毒症综合征爆发的病原体。研究了益生菌剂丁酸梭菌MIYAIRI菌株588对肠出血性大肠杆菌O157:147感染的体外和体内作用。与丁酸梭菌共培养可抑制肠出血性大肠杆菌01 57:H7的生长和志贺样毒素的产生。丁酸和乳酸的抑菌效果呈剂量依赖性。此外，丁酸不仅在低pH条件下对肠出血性大肠杆菌的活力有抑制作用，而且在中性pH调节到7.0时也有抑制作用。流式细胞术分析表明，Caco-2细胞与C. butyricum和E. coli K12预孵育可抑制EHEC O157:H7的粘附。而丁酸C对肠出血性大肠杆菌粘附Caco-2细胞的抑制作用强于大肠杆菌K12。感染肠出血性大肠杆菌O157:H7的小鼠在感染后4-7天内死亡。另一方面，所有经预防性丁酸梭菌预处理的无糖生物小鼠暴露于EHEC O157:H7后均存活，而经治疗性丁酸梭菌预处理的无糖生物小鼠中有50%存活。与肠出血性大肠杆菌O157:H7和丁酸梭菌不相关的无菌小鼠粪便内容物中的肠出血性大肠杆菌O157:147计数和志贺样毒素（Stx1和Stx2）的数量均低于与肠出血性大肠杆菌O157:H7不相关的无菌小鼠。上述结果表明，益生菌C - butyricum MIYAIRI菌株588对小鼠肠出血性大肠杆菌O157:1-17感染具有预防和治疗作用。(C) 2004年欧洲微生物学会联合会。Elsevier B.V.版权所有。
Enterohemorrhagic Escherichia coli (EHEC) 0 157:117 has been considered as an agent responsible for outbreak of hemorrhagic colitis and the hemolytic uremic syndrome. We examined the effect of the probiotic agent Clostridium butyricum MIYAIRI strain 588 on EHEC O157:147 infections in vitro and in vivo using gnotobiotic mice. The growth of EHEC 01 57:H7 and the production of Shiga-like toxins in broth cultures were inhibited by co-incubation with C. butyricum. The antibacterial effects of butyric and lactic acid were demonstrated in a dose-dependent manner. In addition, the inhibitory effect of butyric acid on the viability of EHEC was demonstrated not only at low pH, but also at neutral pH adjusted to 7.0. Flowcytometric analysis showed that pre-incubation of Caco-2 cells with C. butyricum and E. coli K12 inhibited the adhesion of EHEC O157:H7. However, the effect of C butyricum on adhesion of EHEC to Caco-2 cells was more inhibitory than that of E. coli K12. Gnotobiotic mice mono-associated with EHEC O157:H7 died within 4-7 days after the infection. On the other hand, all gnotobiotic mice prophylactically pre-treated with C. butyricum survived exposure to EHEC O157:H7 and of the gnotobiotic mice therapeutically post-treated with C. butyricum, 50% survived. Both counts of EHEC O157:147 and the amounts of shiga-like toxins (Stx1 and Stx2) in fecal contents of gnotobiotic mice di-associated with EHEC O157:H7 and C. butyricum were less than those of gnotobiotic mice mono-associated with EHEC O157:H7. These results indicated that the probiotic bacterium C butyricum MIYAIRI strain 588 has preventive and therapeutic effects on EHEC O157:1-17 infection in gnotobiotic mice. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.