Expression profiles of six novel C-type lectins in response to bacterial and 20E injection in the cotton bollworm (Helicoverpa armigera)
Expression profiles of six novel C-type lectins in response to bacterial and 20E injection in the cotton bollworm (Helicoverpa armigera)
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棉铃虫 (Helicoverpa armigera) 中六种新型 C 型凝集素对细菌和 20E 注射的反应的表达谱
DOI：
10.1016/j.dci.2012.04.004
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发表时间：
2012-06-01
影响因子：
2.9
通讯作者：
Wang, Yu-Feng
中科院分区：
文献类型：
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作者：
Wang, Jia-Lin;Liu, Xu-Sheng;Wang, Yu-Feng
c型凝集素可以作为模式识别受体（PRRs），在先天免疫中发挥重要作用。两种c型凝集素（HaCTL1和HaCTL2）已在棉铃虫（Helicoverpa armigera）中发现。本研究从棉蚜中分离出6种c型凝集素（HaCTL3、4、5、6、7和8）。所有六个新的hactl编码一个信号肽（或部分信号肽）和完整的串联碳水化合物识别结构域（CRDs）。注射革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌后，脂肪体中HaCTL4、5、6、7和8 mRNA均升高，而仅注射大肠杆菌后，HaC7L3 mRNA上调。重组HaCTL3对革兰氏阴性菌和革兰氏阳性菌均表现出钙依赖的凝集活性。凝集抑制分析表明，rHaCTL3可识别麦芽糖、海藻糖、肽聚糖和脂多糖。注射20-羟基脱皮激素（20E）后，HaCTL3、HaCTL8 mRNA表达上调，HaCTL4、5、6 mRNA表达下调。我们的研究结果表明，6种新型的c型凝集素可能在棉铃虫抵御细菌PRRs中发挥重要作用，激素20E可能通过凝集素调节免疫功能。(C) 2012 Elsevier Ltd.版权所有。
C-type lectins can act as pattern recognition receptors (PRRs) and play an important role in innate immunity. Two C-type lectins (HaCTL1 and HaCTL2) have been previously identified in the cotton bollworm (Helicoverpa armigera). Here we isolate six C-type lectins from H. armigera (HaCTL3, 4, 5, 6, 7 and 8). All six new HaCTLs encode a signal peptide (or partial signal peptide) and complete tandem carbohydrate-recognition domains (CRDs). HaCTL4, 5, 6, 7 and 8 mRNA increased in the fat body after injection with both killed Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, whereas HaC7L3 mRNA was upregulated following E. coli injection only. Recombinant HaCTL3 exhibited agglutinating activity against both Gram-negative and Gram-positive bacteria in a calcium-dependent manner. Agglutination inhibitory analysis indicated that rHaCTL3 recognizes maltose, trehalose, peptidoglycan and lipopolysaccharides. HaCTL3 and HaCTL8 mRNA showed upregulation while HaCTL4, 5, and 6 mRNA downregulation post 20-Hydroxyecdysone (20E) injection. Our results indicate that the six novel C-type lectins of H. armigera may play important roles in defending against bacteria as PRRs and the hormone 20E can function in regulating immunity through lectins. (C) 2012 Elsevier Ltd. All rights reserved.