Blue light-induced reactivity of retinal age pigment. In vitro generation of oxygen-reactive species.
Blue light-induced reactivity of retinal age pigment. In vitro generation of oxygen-reactive species.
复制标题
DOI：
10.1097/00006982-199616040-00023
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
M. Różanowska;J. Jarvis-Evans;W. Korytowski;M. Boulton;J. Burke;T. Sarna
中科院分区：
文献类型：
--
作者：
M. Różanowska;J. Jarvis-Evans;W. Korytowski;M. Boulton;J. Burke;T. Sarna
眼睛暴露在强光下，特别是蓝光，会对视网膜造成不可逆的氧依赖性损伤。然而，还没有发现触发这种光氧化过程的关键发色团。我们发现，用光照射人类视网膜色素上皮（RPE）细胞会诱导显着的氧气吸收，这与波长和年龄有关。RPE细胞及其老化色素脂褐素的光反应性的分析表明，在RPE细胞中观察到的光反应性主要是由于脂褐素的存在，其在有氧条件下产生几种氧反应性物质，包括单线态氧、超氧阴离子和过氧化氢。我们还发现，脂褐素光敏需氧反应导致增强的脂质过氧化作用，如通过脂质过氧化氢和丙二醛在光照的色素颗粒中的积累所测量的。过氧化氢只是脂褐素有氧光激发的次要产物。我们推测脂褐素是一种潜在的光敏剂，可能会增加视网膜光损伤的风险，并有助于年龄相关性黄斑病变的发展。
Exposure of the eye to intense light, particularly blue light, can cause irreversible, oxygen-dependent damage to the retina. However, no key chromophores that trigger such photooxidative processes have been identified yet. We have found that illumination of human retinal pigment epithelium (RPE) cells with light induces significant uptake of oxygen that is both wavelength- and age-dependent. Analysis of photoreactivity of RPE cells and their age pigment lipofuscin indicates that the observed photoreactivity in RPE cells is primarily due to the presence of lipofuscin, which, under aerobic conditions, generates several oxygen-reactive species including singlet oxygen, superoxide anion, and hydrogen peroxide. We have also found that lipofuscin-photosensitized aerobic reactions lead to enhanced lipid peroxidation as measured by accumulation of lipid hydroperoxides and malondialdehyde in illuminated pigment granules. Hydrogen peroxide is only a minor product of aerobic photoexcitation of lipofuscin. We postulate that lipofuscin is a potential photosensitizer that may increase the risk of retinal photodamage and contribute to the development of age-related maculopathy.