Ebola RNA Persistence in Semen of Ebola Virus Disease Survivors - Final Report.
Ebola RNA Persistence in Semen of Ebola Virus Disease Survivors - Final Report.
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DOI：
10.1056/nejmoa1511410
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发表时间：
2017-10-12
期刊：
影响因子：
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通讯作者：
Sahr F
中科院分区：
文献类型：
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作者：
Deen GF;Broutet N;Xu W;Knust B;Sesay FR;McDonald SLR;Ervin E;Marrinan JE;Gaillard P;Habib N;Liu H;Liu W;Thorson AE;Yamba F;Massaquoi TA;James F;Ariyarajah A;Ross C;Bernstein K;Coursier A;Klena J;Carino M;Wurie AH;Zhang Y;Dumbuya MS;Abad N;Idriss B;Wi T;Bennett SD;Davies T;Ebrahim FK;Meites E;Naidoo D;Smith SJ;Ongpin P;Malik T;Banerjee A;Erickson BR;Liu Y;Liu Y;Xu K;Brault A;Durski KN;Winter J;Sealy T;Nichol ST;Lamunu M;Bangura J;Landoulsi S;Jambai A;Morgan O;Wu G;Liang M;Su Q;Lan Y;Hao Y;Formenty P;Ströher U;Sahr F
埃博拉病毒在男性从埃博拉病毒病（EVD）中康复后的精液中被检测到。我们报告了塞拉利昂一组EVD幸存者精液中存在埃博拉病毒RNA。我们在塞拉利昂从埃博拉治疗单位（ETU）出院后的不同时间，分两个阶段招募了220名EVD成年男性幸存者的便利样本（第1阶段有100名参与者，第2阶段有120名参与者）。通过使用NP和VP 40（第1阶段）或NP和GP（第2阶段）的靶序列进行定量逆转录酶聚合酶链反应（RT-PCR）测定，对基线时获得的精液标本进行检测。本研究并未直接评估EVD性传播的风险。在210名提供初始精液样本进行分析的参与者中，57名（27%）在定量RT-PCR中获得阳性结果。在所有7名男子的精液中检测到埃博拉病毒RNA，其标本在ETU出院后3个月内获得，42名男子中有26名（62%）在4至6个月获得，60名男子中有15名（25%）在7至9个月获得，26名男子中有4名（15%）在10至12个月获得，在13至15个月时获得标本的38名男性中有4名（11%），在16至18个月时获得标本的25名男性中有1名（4%），在19个月或更晚时获得标本的男性中没有。在第1阶段有阳性结果的46名参与者中，在ETU出院后3个月内，NP和VP 40靶标的RNA值（较高的值表示较低的RNA值）较低（分别为32.4和31.3;在7名男性中）比4至6个月时（34.3和33.1; 25例）、7 - 9个月（37.4和36.6; 13例）和10 - 12个月（37.7和36.9; 1例）。在第2阶段，共有11名参与者的NP和GP目标结果呈阳性（在ETU出院后4.1至15.7个月获得样本）; NP的周期阈值范围为32.7至38.0，GP为31.1至37.7。这些数据表明，埃博拉病毒RNA在精液中的长期存在，并随着ETU排出后时间的增加而持续下降。（由世界卫生组织和其他组织资助。
Ebola virus has been detected in the semen of men after their recovery from Ebola virus disease (EVD). We report the presence of Ebola virus RNA in semen in a cohort of survivors of EVD in Sierra Leone. We enrolled a convenience sample of 220 adult male survivors of EVD in Sierra Leone, at various times after discharge from an Ebola treatment unit (ETU), in two phases (100 participants were in phase 1, and 120 in phase 2). Semen specimens obtained at baseline were tested by means of a quantitative reverse-transcriptase–polymerase-chain-reaction (RT-PCR) assay with the use of the target sequences of NP and VP40 (in phase 1) or NP and GP (in phase 2). This study did not evaluate directly the risk of sexual transmission of EVD. Of 210 participants who provided an initial semen specimen for analysis, 57 (27%) had positive results on quantitative RT-PCR. Ebola virus RNA was detected in the semen of all 7 men with a specimen obtained within 3 months after ETU discharge, in 26 of 42 (62%) with a specimen obtained at 4 to 6 months, in 15 of 60 (25%) with a specimen obtained at 7 to 9 months, in 4 of 26 (15%) with a specimen obtained at 10 to 12 months, in 4 of 38 (11%) with a specimen obtained at 13 to 15 months, in 1 of 25 (4%) with a specimen obtained at 16 to 18 months, and in no men with a specimen obtained at 19 months or later. Among the 46 participants with a positive result in phase 1, the median baseline cycle-threshold values (higher values indicate lower RNA values) for the NP and VP40 targets were lower within 3 months after ETU discharge (32.4 and 31.3, respectively; in 7 men) than at 4 to 6 months (34.3 and 33.1; in 25), at 7 to 9 months (37.4 and 36.6; in 13), and at 10 to 12 months (37.7 and 36.9; in 1). In phase 2, a total of 11 participants had positive results for NP and GP targets (samples obtained at 4.1 to 15.7 months after ETU discharge); cycle-threshold values ranged from 32.7 to 38.0 for NP and from 31.1 to 37.7 for GP. These data showed the long-term presence of Ebola virus RNA in semen and declining persistence with increasing time after ETU discharge. (Funded by the World Health Organization and others.)