Silk Fibroin Scaffold as a Potential Choice for Female Pelvic Reconstruction: A Study on the Biocompatibility in Abdominal Wall, Pelvic, and Vagina
Silk Fibroin Scaffold as a Potential Choice for Female Pelvic Reconstruction: A Study on the Biocompatibility in Abdominal Wall, Pelvic, and Vagina
复制标题
丝素蛋白支架作为女性盆腔重建的潜在选择：腹壁、盆腔和阴道生物相容性研究
DOI：
10.1002/jemt.22653
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发表时间：
2017-03-01
影响因子：
2.5
通讯作者：
Wang, Jianliu
中科院分区：
文献类型：
--
作者：
Chang, Yue;Sun, Xiuli;Wang, Jianliu
本研究的目的是比较大鼠腹壁、阴道和盆腔膀胱子宫对丝素蛋白支架的组织反应。将丝素蛋白支架皮下植入 16 只大鼠的腹部、盆腔膀胱子宫间隙和阴道粘膜下。术后2周、4周、8周和12周对动物实施安乐死。进行苏木精和伊红染色以评估细胞浸润、支架内部和周围的巨噬细胞和粒细胞的百分比。通过免疫荧光测定评估支架和宿主组织界面处的 M1/M2 巨噬细胞的数量。三组急性炎症程度相似，持续时间不超过4周。在腹部植入的丝素蛋白支架中发现成纤维细胞向内生长更快，而阴道植入的支架则比盆腔和腹部组的组织向内生长速度更慢并且巨噬细胞浸润更多。然而，三组中的 M2 细胞数量显着增加。一般来说，丝素蛋白在腹部、阴道和盆腔组织中具有良好的生物相容性，能够促进健康组织的形成，可能是女性盆腔重建的潜在选择。 (C) 2016 年 Wiley 期刊公司。
The aim of this study was to compare the tissue reactions to silk fibroin scaffolds in the abdominal wall, vagina, and pelvic vesico-uterine of rats. Silk fibroin scaffolds were implanted subcutaneously in the abdominal, pelvic vesico-uterine space, and under the vaginal mucosa of 16 rats. The animals were euthanized at 2, 4, 8, and 12 weeks postoperatively. Hematoxylin and eosin staining was performed to evaluate cellular infiltration, the percentage of macrophages and granulocytes inside and around the scaffolds. The amounts of M1/M2 macrophages at the interface of scaffolds and host tissue were evaluated through an immunofluorescence assay. The degree of acute inflammation was similar among the three groups, and lasted no more than 4 weeks. A faster ingrowth of fibroblasts was found in the abdominally implanted silk fibroin scaffolds, while vaginal implanted scaffolds committed a slower tissue ingrowth rate and much more macrophages infiltration than the pelvic and abdominal group. However, a significantly higher amount of M2 cells were seen in the three groups. In general, silk fibroin has nice biocompatibility in the abdominal, vagina, and pelvic tissue, eliciting healthy tissue formation, and might be a potential choice for female pelvic reconstruction. (C) 2016 Wiley Periodicals, Inc.