Quantitation of specific parameters of motility in large numbers of human sperm by digital image processing.
Quantitation of specific parameters of motility in large numbers of human sperm by digital image processing.
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通过数字图像处理对大量人类精子的特定运动参数进行定量。
DOI：
10.1095/biolreprod38.2.270
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发表时间：
1988
影响因子：
3.6
通讯作者：
Tash,JS
中科院分区：
文献类型：
--
作者：
Mack,SO;Wolf,DP;Tash,JS
CellSoftTM计算机辅助数字图像分析系统经验证可用于定量大量人类精子的特定运动参数。从线性头部轨迹（1型）到具有重叠轨迹的非线性、不对称模式（5型）的运动模式在精液和制备用于体外受精的洗涤样本中进行主观鉴定。每种类型的代表用于优化数字成像设置参数、跟踪速率和频率。还根据以下运动参数表征每种细胞类型：曲线速度（Vcl）、直线速度（Vsl）、向前进展的线性度（Lin）、最大和平均侧头振幅（maxLHA;平均LHA）和交叉搏动频率（BCF）。对所有可通过数字和手动测定的参数（Vcl、Vsl、Lin和BCF）进行比较，结果表明Vcl、Lin或BCF无差异（p > 0.05），Vsl测量值仅存在轻微差异（5-6%）。数字成像技术验证后，研究了精液和洗涤细胞的群体。相同样品的重复分析证明无显著的试验内变异性。对来自10个不同供体的精液和洗涤细胞的比较表明，除Lin外，洗涤细胞的所有运动参数均显著较高（p < 0.05）。得出的结论是，数字成像系统可以充分和快速定量大量的细胞与异质运动模式。这项技术可能被证明是有用的定义与获能，顶体反应，和人类精子的生育力的运动特性。
The CellSoftTMcomputer-assisted digital image analysis system was validated for quantitating specific motility parameters in large numbers of human sperm. Motility patterns ranging from linear head trajectories (Type 1) to nonlinear, asymmetric patterns with overlapping trajectory (Type 5) were subjectively identified in semen and washed samples prepared for in vitro fertilization. A representative of each type was used for optimizing the digital imaging set-up parameters, tracking rate, and frequency. Each cell type was also characterized according to the following motility parameters: curvilinear velocity (Vcl), straight line velocity (Vsl), linearity of forward progression (Lin), maximum and mean lateral head amplitude (maxLHA; mean LHA), and beat cross frequency (BCF). Comparison of all parameters that could be determined both digitally and manually (Vcl, Vsl, Lin, and BCF) indicated no differences (p > 0.05) in Vcl, Lin, or BCF and only slight differences (5–6%) in Vsl measurements. After validation of the digital imaging technique, populations of seminal and washed cells were studied. Replicate analysis of the same sample demonstrated no significant intraassay variability. A comparison of semen and washed cells from 10 different donors indicated that all of the motility parameters, with the exception of Lin, were significantly higher (p < 0.05) in washed cells. It was concluded that the digital imaging system can adequately and rapidly quantitate a large number of cells with heterogeneous motility patterns. This technique may prove to be useful in defining motility characteristics associated with capacitation, the acrosome reaction, and fertility of human sperm.