Combination of amino acids in the 3a protein and the coat protein of Cucumber mosaic virus determines symptom expression and viral spread in bottle gourd
Combination of amino acids in the 3a protein and the coat protein of Cucumber mosaic virus determines symptom expression and viral spread in bottle gourd
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黄瓜花叶病毒3a蛋白和外壳蛋白中的氨基酸组合决定了葫芦中的症状表达和病毒传播
DOI：
10.1007/s007050170140
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发表时间：
2001
影响因子：
2.7
通讯作者：
Y. Takanami
中科院分区：
文献类型：
--
作者：
M. Takeshita;M. Suzuki;Y. Takanami
摘要：感染黄瓜花叶病毒分离物( -KM)的葫芦植株表现出严重的慢性花叶症状(SCM)并伴有矮化，而另外两个分离物(CMV-Y和CMV-D8)则没有。CMV-KM和CMV-D8引起的黄化斑点扩大并迅速扩散到接种的子叶上，而CMV-Y则引起过敏性反应(HR)产生点状坏死灶。3个分离物之间的重新组合分析表明，植物上的局部和系统症状受到RNA3的调控。3个RNA3基因的反向重组和定点突变分析表明，运动蛋白(MP)基因和外壳蛋白(CP)基因编码的遗传信息共同决定了丝瓜细胞质雄性不育的诱导。当CMV-D8的MP中的Ser51被改变为Asn51时发生SCMS，而在CMV-KM的MP中Ser51替换Asn51使其丧失了诱导SCMS的能力。此外，CPS中的Ser129被证明负责HR的诱导以及有效的细胞到细胞和长距离运动的阻断。
Summary. Bottle gourd plants infected with an isolate of cucumber mosaic virus (CMV-KM) developed severe chronic mosaic symptoms (SCMS) with stunting, but two other isolates (CMV-Y and CMV-D8) did not. CMV-KM and CMV-D8 induced enlarged chlorotic spots and rapidly spread over the inoculated cotyledons, whereas CMV-Y elicited a hypersensitive response (HR) producing pin-point necrotic lesions. Reassortment analysis among the three isolates revealed that the local and systemic symptoms on the plants were regulated by RNA3. Reciprocal recombination and site-directed point mutation analyses of the three RNA3s demonstrated that a combination of genetic information encoded by the movement protein (MP) gene and the coat protein (CP) gene determines the induction of SCMS in bottle gourd. SCMS occurred when Ser51 in the MP of CMV-D8 was changed to Asn51, whereas substitution of Ser51 for Asn51 in the MP of CMV-KM eliminated its ability to induce SCMS. Furthermore, Ser129 in the CPs was shown to be responsible for induction of HR and blocking of efficient cell-to-cell and long-distance movement.