Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles
Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles
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DOI：
10.1016/s0378-5173(02)00548-3
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发表时间：
2003-01-02
影响因子：
5.8
通讯作者：
Du, YM
中科院分区：
文献类型：
--
作者：
Xu, YM;Du, YM
利用三聚磷酸钠（TPP）和壳聚糖的离子凝胶化过程制备了不同形态的壳聚糖纳米粒（CS NP）。用透射电镜观察了它们的直径为20-200 nm和球形。FTIR证实了纳米颗粒中TPP的三聚磷酸基团与壳聚糖的铵基团连接。以牛血清白蛋白（BSA）为模型蛋白，考察了壳聚糖的分子量（Mw）、脱乙酰度（DD）、壳聚糖浓度、BSA初始浓度以及包封介质中聚乙二醇（PEG）的存在等因素对包封性能的影响。当壳聚糖分子量从10 kDa增加到210 kDa时，BSA的包封率提高了约2倍，BSA在pH 7.4的PBS（磷酸盐缓冲液）中8天的总释放量从73.9%降低到17.6%。将DD从75.5%增加到92%，略微提高了包封率并减慢了释放速率。包封率随BSA和壳聚糖初始浓度的增加而显著降低;较高的BSA负载量加快了BSA从纳米粒中的释放。PEG的加入阻碍了BSA的包封，加快了释放速率。(C)2002 Elsevier Science B. V.保留所有权利。
Chitosan nanoparticles (CS NP) with various formations were produced based on ionic gelation process of tripolyphosphate (TPP) and chitosan. They were examined with diameter 20-200 nm and spherical shape using TEM. FTIR confirmed tripolyphosphoric groups of TPP linked with ammonium groups of chitosan in the nanoparticles. Factors affecting delivery properties of bovine serum albumin (BSA) as model protein have been tested, they included molecular weight (Mw) and deacetylation degree (DD) of chitosan, the concentration of chitosan and initial BSA, and the presence of polyethylene glycol (PEG) in encapsulation medium. Increasing Mws of chitosan from 10 to 210 kDa, BSA encapsulation efficiency was enhanced about two times, BSA total release in PBS (phosphate buffer saline) pH 7.4 in 8 days was reduced from 73.9 to 17.6%. Increasing DD from 75.5 to 92% promoted slightly the encapsulation efficiency and decelerated the release rate. The encapsulation efficiency was highly decreased by increase of initial BSA and chitosan concentration; higher loading capacity of BSA speeded the BSA release from the nanoparticles. Adding PEG hindered the BSA encapsulation and accelerated the release rate. (C) 2002 Elsevier Science B.V. All rights reserved.