A novel dataset for identifying sex-biased genes in Drosophila.
A novel dataset for identifying sex-biased genes in Drosophila.
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DOI：
10.7150/jgen.7955
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发表时间：
2014
影响因子：
--
通讯作者：
Vibranovski MD
中科院分区：
文献类型：
--
作者：
VanKuren NW;Vibranovski MD
两性二态物种的雄性和雌性之间的表型差异在很大程度上是由性别之间的基因表达差异造成的，其中大部分发生在性腺中。为了准确地鉴定果蝇雄性和雌性之间差异表达的基因，我们对果蝇的睾丸和卵巢转录进行了测序，并利用它们来鉴定后两个物种中的性别偏见基因。我们强调，与男性和女性全身转录组数据相比，使用我们的睾丸/卵巢数据时，性别偏见基因检测方法的灵敏度和改进的能力都有所提高。因此，我们提供了一个专门设计的资源，以准确地识别和表征整个果蝇的性别偏见基因。该数据集可通过NCBI GEO加入GSE52058获得。
Phenotypic differences between males and females of sexually dimorphic species are caused in large part by differences in gene expression between the sexes, most of which occurs in the gonads. To accurately identify genes differentially expressed between males and females in Drosophila, we sequenced the testis and ovary transcriptomes of D. yakuba, D. pseudoobscura, and D. ananassae and used them to identify sex-biased genes in the latter two species. We highlight the increased sensitivity and improved power of sex-biased gene detection methods when using our testis/ovary data versus male and female whole body transcriptome data. We thus provide a resource specifically designed to accurately identify and characterize sex-biased genes across Drosophila. This dataset is available through NCBI GEO accession GSE52058.