Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade
Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade
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DOI：
10.1016/j.bbrc.2009.02.011
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发表时间：
2009-03-27
影响因子：
3.1
通讯作者：
Fujimoto, Masafumi
中科院分区：
文献类型：
--
作者：
Kimura, Ikuo;Konishi, Morichika;Fujimoto, Masafumi
成年小鼠在白色脂肪组织中大量表达neudesin，一种具有神经营养活性的细胞外血红素结合蛋白。然而，在脂肪细胞分化的早期阶段，在脂肪形成过程中，neudesin的表达短暂下降。Neudesin-hemin显著抑制3 T3-L1细胞的脂肪形成。通过RNA干扰敲低neudesin显著促进3 T3-L1细胞中的脂肪形成，并降低脂肪细胞分化过程中MAPK的活化。添加或敲低neudesin影响C/EBP α和PPAR γ的表达，但不影响C/EBP β的表达。这些发现表明，neudesin在脂肪细胞分化的早期阶段中起着关键作用，其中C/EBP β通过控制MAPK途径诱导PPAR γ和C/EBP α表达。(C)2009爱思唯尔公司All rights reserved.
Adult mice abundantly express neudesin, an extracellular heme-binding protein with neurotrophic activity, in white adipose tissues. At the early stage of adipocyte differentiation during adipogenesis, however, the expression of neudesin decreased transiently. Neudesin-hemin significantly suppressed adipogenesis in 3T3-L1 cells. The knockdown of neudesin by RNA interference markedly promoted adipogenesis in 3T3-L1 cells and decreased MAPK activation during adipocyte differentiation. The addition or knockdown of neudesin affected the expression of C/EBP alpha and PPAR gamma but not of C/EBP beta. These findings suggest that neudesin plays a critical role in the early stage of adipocyte differentiation in which C/EBP beta induces PPAR gamma and C/EBP alpha expressions, by controlling the MAPK pathway. (C) 2009 Elsevier Inc. All rights reserved.