Golgi 58K-like protein in pollens and pollen tubes ofLilium davidii
Golgi 58K-like protein in pollens and pollen tubes ofLilium davidii
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DOI：
10.1007/bf02879305
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发表时间：
2000-08
期刊：
影响因子：
--
通讯作者：
Yan Li;Longfei Yan
中科院分区：
文献类型：
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作者：
Yan Li;Longfei Yan
在动物细胞中，高尔基体位于微管组织中心（MTOC）附近，其位置部分由58K蛋白决定。利用十二烷基硫酸钠凝胶电泳（SDS-PAGE）和免疫印迹法，在大叶百合的花粉粒和花粉管中发现了一种58k样蛋白。其分子量与动物细胞中的58K蛋白非常相似。通过免疫荧光标记，在共聚焦激光扫描显微镜（CLSM）下，动物58K抗体在花粉粒和花粉管中可见点状染色，这与植物细胞中高尔基体的分布一致。此外，免疫金标和透射电镜显示，58k样蛋白主要结合在高尔基体附近的囊泡样结构膜上。这是58k样蛋白首次在植物细胞中得到证实。
In animal cells, Golgi apparatus is located near the microtubule organizing center (MTOC) and its position is determined partly by 58K protein. By sodium dodecyl-sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and immuno-blotting methods, a 58K-like protein has been found in pollen grains and pollen tubes ofLilium davidii. Its molecular weight is very similar to that of the 58K protein of animal cells. By immunofluorescence labeling, under a confocal laser scanning microscope (CLSM), the animal 58K antibody revealed a punctate staining in pollen grains and pollen tubes, which is consistent with the distribution of Golgi apparatus in plant cells. In addition, immuno-gold labeling and transmission electron microscopy showed that the 58K-like protein bound mainly to the membrane of vesicles-like structure near Golgi apparatus. This is the first demonstration of the 58K-like protein in plant cells.