Comprehensive comparison brassinosteroid-regulated of auxin-regulated and brassinosteroid-regulated genes in arabidopsis
Comprehensive comparison brassinosteroid-regulated of auxin-regulated and brassinosteroid-regulated genes in arabidopsis
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DOI：
10.1104/pp.103.034736
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发表时间：
2004-04-01
期刊：
影响因子：
7.4
通讯作者：
Yoshida, S
中科院分区：
文献类型：
--
作者：
Goda, H;Sawa, S;Yoshida, S
虽然许多生理学研究已经解决了油菜素类固醇和生长素之间的相互作用，很少有人知道有关的分子机制。利用代表约8，300个拟南芥基因的Affyssin基因芯片，我们研究了吲哚-3-乙酸（IAA）和油菜素（BL）在24小时内的全面转录谱。我们鉴定了409个BL诱导基因，276个IAA诱导基因，总共637个基因。这两种激素只调节了48个共同的基因，这表明每种激素的大部分作用都是由各自独特的基因表达介导的。IAA上调基因在共同调控基因中富集。它们被IAA诱导得很快，被BL诱导得更慢，这表明它们具有不同的生理作用。许多是早期生长素诱导基因及其同源物，即SAUR，GH 3和IAA。综合比较还确定了IAA和BL特异性基因，这将有助于阐明每种激素的具体作用。基于它们对两种激素的反应的相似性，使用层次聚类对所识别的基因进行分类。基因分类也使我们能够分析顺式元件的频率。TGTCTC元件是先前报道的生长素反应元件的核心元件，其不富集由IAA特异性调控的基因，而是富集在由IAA和BL均上调的基因的5 '侧翼区。这样的基因分类应该是有用的预测未知基因的功能，了解这两种激素的作用，和启动子分析应该提供洞察到转录调控的两种激素的相互作用。
Although numerous physiological studies have addressed the interactions between brassinosteroids and auxins, little is known about the underlying molecular mechanisms. Using an Affymetrix GeneChip representing approximately 8,300 Arabidopsis genes, we studied comprehensive transcript profiles over 24 h in response to indole-3-acetic acid (IAA) and brassinolide (BL). We identified 409 genes as BL inducible, 276 genes as IAA inducible, and 637 genes in total. These two hormones regulated only 48 genes in common, suggesting that most of the actions of each hormone are mediated by gene expression that is unique to each. IAA-up-regulated genes were enriched in genes regulated in common. They were induced quickly by IAA and more slowly by BL, suggesting divergent physiological roles. Many were early auxin-inducible genes and their homologs, namely SAUR, GH3, and IAA. The comprehensive comparison also identified IAA- and BL-specific genes, which should help to elucidate the specific actions of each hormone. The identified genes were classified using hierarchical clustering based on the similarity of their responses to the two hormones. Gene classification also allowed us to analyze the frequency of cis-elements. The TGTCTC element, a core element of the previously reported auxin response element, was not enriched in genes specifically regulated by IAA but was enriched in the 5'-flanking region of genes up-regulated by both IAA and BL. Such gene classification should be useful for predicting the functions of unknown genes, to understand the roles of these two hormones, and the promoter analysis should provide insight into the interaction of transcriptional regulation by the two hormones.