Proteins are polyisoprenylated in Arabidopsis thaliana
Proteins are polyisoprenylated in Arabidopsis thaliana
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DOI：
10.1016/j.bbrc.2004.08.025
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发表时间：
2004-09-24
影响因子：
3.1
通讯作者：
Swiezewska, E
中科院分区：
文献类型：
--
作者：
Gutkowska, M;Bienkowski, T;Swiezewska, E
发现类异戊二烯脂质与拟南芥的蛋白质共价连接。其鉴别（聚戊烯醇：通过HPLC/ESI-MS，应用多反应监测技术以及用[H-3]甲羟戊酸和其他前体对拟南芥植物的代谢标记，证实了Prenol-9-11和Pren-10占主导地位和dolichols：Dol-15-17和Dol-16占主导地位。还注意到典型的法尼醇、香叶基香叶醇和植醇修饰的蛋白质的发生。放射性同位素标记允许检测共价结合到甲羟戊酸衍生的类异戊二烯醇的几种蛋白质。一个显着的部分聚异戊二烯化的蛋白质被回收在拟南芥细胞的胞质/轻囊泡馏分亚分级分离后。总之，我们的数据证明，植物蛋白质的一个子集是聚异戊二烯。(C)2004年由Elsevier Inc.出版
Isoprenoid lipids were found to be covalently linked to proteins of Arabidopsis thaliana. Their identity (polyprenols: Prenol-9-11 with Pren-10 dominating and dolichols: Dol-15-17 with Dol-16 dominating) was confirmed by means of HPLC/ESI-MS with application of the multiple reaction monitoring technique as well as metabolic labeling of Arabidopsis plants with [H-3]mevalonate and other precursors. The occurrence of typical farnesol-, geranylgeraniol-, and phytol-modified proteins was also noted. Radioisotopic labeling allowed detection of several proteins that were covalently bound to mevalonate-derived isoprenoid alcohols. A significant portion of polyisoprenylated proteins was recovered in the cytosolic/light vesicular fraction of Arabidopsis cells upon subfractionation. Taken together our data prove that a subset of plant proteins is polyisoprenylated. (C) 2004 Published by Elsevier Inc.