SEQUENCE DETERMINATION AND GENETIC CONTENT OF THE SHORT UNIQUE REGION IN THE GENOME OF HERPES-SIMPLEX VIRUS TYPE-1
SEQUENCE DETERMINATION AND GENETIC CONTENT OF THE SHORT UNIQUE REGION IN THE GENOME OF HERPES-SIMPLEX VIRUS TYPE-1
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DOI：
10.1016/0022-2836(85)90320-1
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发表时间：
1985-01-01
影响因子：
5.6
通讯作者：
RIXON, FJ
中科院分区：
文献类型：
--
作者：
MCGEOCH, DJ;DOLAN, A;RIXON, FJ
测定了单纯疱疹病毒1型17株基因组短独特区的完整DNA序列，并从mRNA和编码蛋白的角度对其进行了解释。该序列包含不同DNA克隆的长度不同的可变区。大多数分析中使用的克隆给出了12,979个碱基对的唯一短长度。该区域似乎包含12个基因，它们由具有不同启动子的mRNA表达，但可能共享3‘-末端，因此，除2个外，所有的mRNA都属于1或4个3’-‘-共末端家族：79%的序列被认为是多肽编码的。有一对基因的编码序列存在广泛的框外重叠。在短的独特区域编码的蛋白质包括2个即刻早期物种、2个病毒粒子表面糖蛋白和1个DNA结合物种。其中六个基因以前很少或根本没有特征。根据对其编码蛋白质预测的氨基酸序列的性质，可以推断其中几种蛋白质可能是膜相关的。
The complete DNA sequence of the short unique region in the genome of herpes simplex virus type 1, strain 17, was determined and it was interpreted in terms of mRNA and encoded proteins. The sequence contains variable regions whose length differs between DNA clones. The clones used for most of the analysis gave a short unique length of 12,979 base-pairs. This region appears to contain 12 genes, which are expressed by mRNA which have separate promoters, but may share 3''-termination sites, so that all but 2 mRNA belong to 1 or 4 3''-coterminal families: 79% of the sequence is considered to be polypeptide coding. One pair of genes has an extensive out-of-frame overlap of coding sequences. The proteins encoded in the short unique region include 2 immediate-early species, 2 virion surface glycoproteins and a DNA-binding species. Six of the genes have little or no previous characterization. From the nature of the amino acid sequences predicted for their encoded proteins, it was deduced that several of these proteins may be membrane-associated.