Rhizobium flavum sp. nov., a triazophos-degrading bacterium isolated from soil under the long-term application of triazophos.
Rhizobium flavum sp. nov., a triazophos-degrading bacterium isolated from soil under the long-term application of triazophos.
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DOI：
10.1099/ijs.0.061523-0
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发表时间：
2014-06
影响因子：
2.8
通讯作者：
Tao Gu;Li Sun;Jun Zhang;X. Sui;Shun-Peng Li
中科院分区：
文献类型：
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作者：
Tao Gu;Li Sun;Jun Zhang;X. Sui;Shun-Peng Li
从土壤中分离到一株革兰氏阴性、不运动、浅黄色杆状细菌YW 14（T），并对其进行了多相分类研究。菌株YW 14（T）在3种不同豆科植物上均未结瘤，PCR检测未检测到nifH和nifD基因。菌株YW 14（T）含有Q-10作为主要的泛醌。主要的细胞脂肪酸是C（18：1）ω 7 c。基于16 S rRNA基因序列和7个管家基因（recA、atpD、gln II、gyrB、rpoB、dnaK和thrC）序列的系统发育分析表明，菌株YW 14（T）属于根瘤菌属（Rhizobium）。菌株YW 14（T）与根瘤菌属已知种的模式菌株的16 SrRNA基因序列相似性为93.4-97.3%。菌株YW 14（T）与模式菌株S 123（T）和杨凌根瘤菌CCBAU 71623（T）的DNA-DNA同源性为19.6- 25.7%，表明菌株YW 14（T）与它们在遗传上存在差异。菌株YW 14（T）也可以通过各种表型特征与这些根瘤菌属的近缘种区分开来。根据菌株YW 14（T）的表型特征、系统发育特征和遗传数据，认为该菌株代表根瘤菌属的一个新种，并建议将其命名为Rhizobium flavum sp. nov.。模式菌株为YW 14（T）（= KACC 17222（T）= CCTCC AB 2013042（T））。
A Gram-stain-negative, non-motile, pale yellow, rod-shaped bacterial strain, YW14(T), was isolated from soil and its taxonomic position was investigated by a polyphasic study. Strain YW14(T) did not form nodules on three different legumes, and the nodD and nifH genes were not detected by PCR. Strain YW14(T) contained Q-10 as the predominant ubiquinone. The major cellular fatty acid was C(18 : 1)ω7c. Phylogenetic analyses based on 16S rRNA gene sequences and seven housekeeping gene sequences (recA, atpD, glnII, gyrB, rpoB, dnaK and thrC) showed that strain YW14(T) belonged to the genus Rhizobium. Strain YW14(T) showed 16S rRNA gene sequence similarity of 93.4-97.3% to the type strains of recognized species of the genus Rhizobium. DNA-DNA relatedness between strain YW14(T) and the type strains of Rhizobium sullae IS123(T) and Rhizobium yanglingense CCBAU 71623(T) was 19.6-25.7%, indicating that strain YW14(T) was distinct from them genetically. Strain YW14(T) could also be differentiated from these phylogenetically related species of the genus Rhizobium by various phenotypic properties. On the basis of phenotypic properties, phylogenetic distinctiveness and genetic data, strain YW14(T) is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium flavum sp. nov. is proposed. The type strain is YW14(T) ( = KACC 17222(T) = CCTCC AB2013042(T)).