Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles
Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles
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DOI：
10.1021/nl047996m
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发表时间：
2005-02-01
期刊：
影响因子：
10.8
通讯作者：
Parak, WJ
中科院分区：
文献类型：
--
作者：
Kirchner, C;Liedl, T;Parak, WJ
研究了CdSe和CdSe/ZnS纳米颗粒在巯基丙酸包覆、硅烷化和聚合物包覆等不同表面修饰下的细胞毒性。对于所有病例，给出了在无血清培养基中开始细胞毒性作用的定量值。这些值与显微镜图像相关，其中研究了细胞对颗粒的摄取。我们的数据表明，除了从颗粒中释放有毒的Cd2+离子外，它们的表面化学性质，特别是它们的聚集稳定性，在细胞毒性作用中起着重要作用。另外的膜片钳实验研究了通过离子通道的粒子对电流的影响。
Cytotoxicity of CdSe and CdSe/ZnS nanoparticles has been investigated for different surface modifications such as coating with mercaptopropionic acid, silanization, and polymer coating. For all cases, quantitative values for the onset of cytotoxic effects in serum-free culture media are given. These values are correlated with microscope images in which the uptake of the particles by the cells has been investigated. Our data suggest that in addition to the release of toxic Cd2+ ions from the particles also their surface chemistry, in particular their stability toward aggregation, plays an important role for cytotoxic effects. Additional patch clamp experiments investigate effects of the particles on currents through ion channels.