Proteomics analysis reveals insight into the mechanism of H-Ras-mediated transformation
Proteomics analysis reveals insight into the mechanism of H-Ras-mediated transformation
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蛋白质组学分析揭示了 H-Ras 介导的转化机制。
DOI：
10.1021/pr060283f
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发表时间：
2006-10-06
影响因子：
4.4
通讯作者：
Zhang, Xue-Min
中科院分区：
文献类型：
--
作者：
Jin, Bao-Feng;He, Kun;Zhang, Xue-Min
我们实施了一个蛋白质组学的方法来系统分析的NIH 3 T3细胞的致癌H-RasV 12转化的蛋白质组的变化。44个蛋白质与Ras介导的转化已被确定，和28个蛋白质以前没有报道。RT-PCR分析表明，约44%的目标蛋白鉴定显示伴随的mRNA丰度的变化。一个主要的发现是gankyrin的上调，这是第一个证据表明，gankyrin途径参与Ras激活的转化。
We implemented a proteomics approach to the systematical analysis of the alterations in the proteome of NIH3T3 cells transformed by oncogenic H-RasV12. Forty-four proteins associated with Ras-mediated transformation have been identified, and 28 proteins were not previously reported. RT-PCR analysis showed that approximately 44% of target proteins identified showed concomitant changes in mRNA abundance. A principal finding was the up-regulation of gankyrin, which was the first evidence to show that gankyrin pathway was implicated in Ras-activated transformation.