Solute transport in the deep and calcified zones of articular cartilage
Solute transport in the deep and calcified zones of articular cartilage
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DOI：
10.1016/j.joca.2007.10.001
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发表时间：
2008-06-01
影响因子：
7
通讯作者：
Winlove, C. P.
中科院分区：
文献类型：
--
作者：
Arkill, K. P.;Winlove, C. P.
目的：（1）确定潮标和钙化软骨是否可渗透低分子量溶质，从而为深层软骨中的细胞提供营养的潜在途径。(2)调查运输从subchonelum微循环到钙化软骨在一个完整的灌注joint和运输的静态loading.Methods的影响：潮标和钙化软骨的渗透性进行了研究，在塞的软骨和subchonelum骨形成的膜的扩散细胞。在一个完整的灌注关节中研究了从软骨下微循环的运输和负荷的影响。两种制剂均使用成熟马的掌指关节，并使用荧光素和罗丹明（m.w.结果：钙化软骨对这两种溶质均具有渗透性，无论是从表面还是从软骨下表面。两种溶质的有效扩散系数都是9 × 10（-9）cm（2）s（-1）的量级，比未钙化软骨中的扩散系数小5倍。钙化区是不均匀的，在潮标附近的两种示踪剂的高摄取。罗丹明B的分布体积高于荧光素，与钙化基质中的显著阴离子电荷一致。静态负荷的完整的关节并没有影响罗丹明B的运输，但引起荧光素的浓度显着下降，无论是在表面和深层zones of the tissue.Conclusions：钙化软骨是渗透到小溶质和软骨下循环可能作出重大贡献的营养深层软骨在成熟的马。静态负荷减少了完整关节中小阴离子溶质的摄取。(C)2007年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objectives: (1) To establish whether the tidemark and calcified cartilage are permeable to low molecular weight solutes, thereby providing a potential pathway for nutrition of cells in the deep cartilage. (2) To investigate transport from the subchondral microcirculation into calcified cartilage in an intact perfused joint and the effects on transport of static loading.Methods: The permeability of the tidemark and calcified cartilage was investigated in plugs of cartilage and subchondral bone which formed the membrane of a diffusion cell. Transport from the subchondral microcirculation and the effects of load were studied in an intact perfused joint. Both preparations used the metacarpophalangeal joints of mature horses and fluorescein and rhodamine (m.w. similar to 400 Da) were employed as tracers, assayed by quantitative fluorescence microscopy on histological sections.Results: Calcified cartilage was permeable to both solutes, both from the superficial and the subchondral sides. The effective diffusivity of both solutes was of the order of 9 x 10(-9) cm(2) s(-1), fivefold less than in the uncalcified cartilage. The calcified zone was heterogeneous, with high uptake of both tracers in the vicinity of the tidemark. The distribution volume of rhodamine B was higher than for fluorescein, consistent with a significant anionic charge in the calcified matrix. Static loading of the intact joint did not affect the transport of rhodamine B but caused a significant decrease in concentration of fluorescein both in the surface and deep zones of the tissue.Conclusions: Calcified cartilage is permeable to small solutes and the subchondral circulation may make a significant contribution to the nutrition of deep cartilage in the mature horse. Static loading reduces the uptake of small anionic solutes in the intact joint. (C) 2007 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.