Early-flowering Scots pines through tissue culture for accelerating tree breeding
Early-flowering Scots pines through tissue culture for accelerating tree breeding
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通过组织培养促进早花欧洲赤松加速树木育种
DOI：
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发表时间：
1996
影响因子：
5.4
通讯作者：
A. Stomp
中科院分区：
文献类型：
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作者：
H. Häggman;T. Aronen;A. Stomp
以欧洲赤松萌发胚的子叶为外植体，通过组织培养获得欧洲赤松植株。最佳的组织培养条件为：1/2GD基本培养基，芽形成期用琼脂凝胶化，生根期用琼脂凝胶化，芽诱导期用5.0μM苄氨基嘌呤（BAP）和0.05 μM萘乙酸（NAA），诱导期2周，生根期用2.7 μM NAA重复脉冲1周。微繁殖的成功是基因型依赖的。平均增殖率各不相同的实验从3至15芽每个胚胎。单胚最大芽产量为35个。生根是繁殖过程中最困难的阶段。在温室中生长时，大部分试管苗具有斜向营养和高度分枝的生长习性。有些个体在3年生时产生大孢子囊孢子叶球，在4年生时产生小孢子囊孢子叶球，花粉有活力。早开花的克隆和保存子叶培养的幼苗的能力为加速树木育种提供了新的可能性。
Scots pine plantlets were produced via tissue culture using cotyledons excised from germinated embryos as explants. The optimum tissue culture conditions were: 1/2GDbasal medium gelled with agar-Gelrite during shoot formation and with agar during rooting, inclusion of 5.0μM benzylaminopurine (BAP) and 0.05 μM naphthaleneacetic acid (NAA) for 2 weeks for shoot induction, and repeated 2.7 μM NAA pulses of 1 week for rooting. Micropropagation success was genotype-dependent. Average multiplication rates varied among experiments from 3 to 15 shoots per embryo. The maximum shoot production from a single embryo was 35. Rooting was the most difficult phase in the propagation process. Most of the plantlets had a plagiotrophic and highly branched growth habit when growing in the greenhouse. Some individuals produced megasporangiate strobili at the age of 3 years and microsporangiate strobili with viable pollen at the age of 4 years. Early-flowering clones and the ability to conserve seedlings from which cotyledons have been cultured give new possibilities for accelerated tree breeding.