Antimicrobial activity of the extracts and physalin D from Physalis alkekengi and evaluation of antioxidant potential of physalin D
Antimicrobial activity of the extracts and physalin D from Physalis alkekengi and evaluation of antioxidant potential of physalin D
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DOI：
10.3109/13880200903062606
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发表时间：
2010-02-01
影响因子：
3.8
通讯作者：
Guvenc, A.
中科院分区：
文献类型：
--
作者：
Helvaci, S.;Kokdil, G.;Guvenc, A.
酸浆（茄科）是欧洲和中国传统民间医学中的一种流行植物，据报道它具有许多民族药理学特性，包括抗真菌、止咳、抗炎、镇痛和退热活性。酸浆属植物的一些活性成分已被研究。然而，尚未对 P. alkekengi 的提取物和酸浆素进行抗菌活性研究。在这项研究中，我们试图确定 P. alkekengi 地上部分的甲醇提取物和植物花萼的二氯甲烷提取物可能的抗菌活性。使用纸片扩散和肉汤微量稀释方法对提取物进行了针对五种革兰氏阳性细菌和五种革兰氏阴性细菌以及五种念珠菌属的测试。使用色谱技术对提取物进行分级分离，以分离出酸浆素，并从提取物中分离出酸浆素D。在H-1-NMR波谱研究的基础上阐明了该化合物的结构，并通过与参考样品和文献数据的比较证实了该化合物的结构。结果表明，所有提取物和酸浆素D均具有抗菌作用，特别是对革兰氏阳性菌，MIC值在32至128μg/mL之间。甲醇提取物对真菌具有中等活性，MIC在128至512μg/mL范围内，但二氯甲烷提取物和酸浆素D对真菌具有低活性，MIC在256至512μg/mL范围内。此外，还通过定性 DPPH（1,1-二苯基-2-三硝苯肼）自由基和 TBA（硫代巴比妥酸）测定评估了 physalin D 的抗氧化活性。 Physalin D 显示出较低的抗氧化活性，IC50 值 >= 10 +/- 2.1。
Physalis alkekengi L. (Solanaceae) is a popular plant in traditional European and Chinese folk medicine, and it has been reported to have many ethnopharmacological properties including antifungal, anti-cough, anti-inflammatory, analgesic, and febricide activities. Some active components from Physalis species have been investigated. However, no antimicrobial activity studies on extracts and physalins of P. alkekengi have been carried out. In this study, we attempted to identify the possible antimicrobial activities of the methanol extract from aerial parts of P. alkekengi and the dichloromethane extract from calyces of the plant. The extracts were tested against five Gram-positive and five Gram-negative bacteria and five Candida species by using disk diffusion and broth microdilution methods. The extracts were fractionated to isolate physalins using chromatographic techniques, and physalin D was isolated from the extracts. The structure of the compound was elucidated on the basis of H-1-NMR spectroscopic study, and confirmed by comparison with a reference sample and literature data. Results indicated that all the extracts and physalin D were characterized by antibacterial action, especially against Gram-positive bacteria, with MIC values between 32 and 128 mu g/mL. The methanol extract had moderate activity against fungi at MICs ranging from 128 to 512 mu g/mL, but the dichloromethane extract and physalin D had low activity against fungi at MICs ranging from 256 to 512 mu g/mL. Additionally, the antioxidant activity of physalin D was evaluated by qualitative DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and TBA (thiobarbituric acid) assays. Physalin D showed low antioxidant activity with an IC50 value of >= 10 +/- 2.1.