Fosfomycin Addition to Poly(D,L-Lactide) Coating Does Not Affect Prophylaxis Efficacy in Rat Implant-Related Infection Model, But That of Gentamicin Does.
Fosfomycin Addition to Poly(D,L-Lactide) Coating Does Not Affect Prophylaxis Efficacy in Rat Implant-Related Infection Model, But That of Gentamicin Does.
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DOI：
10.1371/journal.pone.0165544
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Bir F
中科院分区：
文献类型：
--
作者：
Gulcu A;Akman A;Demirkan AF;Yorukoglu AC;Kaleli I;Bir F
庆大霉素是用于预防种植体相关感染(IRI)的种植体涂层的首选抗菌剂。然而，目前大量局部和全身使用庆大霉素会导致耐药性增加，这使得其未来的使用以及相关的预防技术变得不确定。磷霉素是一种替代抗菌剂，它缺乏其他类别抗生素所具有的交叉耐药性。我们评价了10%磷霉素包被的聚(D，L-丙交酯)K线在大鼠脑缺血再灌注损伤模型中的预防效果，并与未包被的(对照1)、包被的(对照2)和含有10%庆大霉素的包被的单层包覆组进行了比较。植入不锈钢克氏针，左侧胫骨腔内注入耐甲氧西林金黄色葡萄球菌(ATCC 43300)悬液(103CFU/10μL)。然后，取下克氏针，在胰酶大豆肉汤和5%绵羊血琼脂中培养。取胫骨切片，苏木精-伊红染色，X射线半定量评价。在PDL中加入磷霉素对X线和组织病理学评分没有影响，但加入庆大霉素则降低了这些评分。在X线检查第28天后，加入庆大霉素组显示出保护作用。仅含磷脂层的涂层无保护作用，添加磷霉素可提供20%的保护水平，添加庆大霉素可提供80%的保护。此外，添加庆大霉素组有103个CFU水平增长，而其他组有105个。因此，在PDL中添加磷霉素不会影响预防效果，但添加庆大霉素会影响预防效果。因此，我们不建议在预防IRI的涂层中使用磷霉素作为单一抗菌剂。
Gentamicin is the preferred antimicrobial agent used in implant coating for the prevention of implant-related infections (IRI). However, the present heavy local and systemic administration of gentamicin can lead to increased resistance, which has made its future use uncertain, together with related preventive technologies. Fosfomycin is an alternative antimicrobial agent that lacks the cross-resistance presented by other classes of antibiotics. We evaluated the efficacy of prophylaxis of 10% fosfomycin-containing poly(D,L-lactide) (PDL) coated K-wires in a rat IRI model and compared it with uncoated (Control 1), PDL-coated (Control 2), and 10% gentamicin-containing PDL-coated groups with a single layer of coating. Stainless steel K-wires were implanted and methicillin-resistant Staphylococcus aureus (ATCC 43300) suspensions (103 CFU/10 μl) were injected into a cavity in the left tibiae. Thereafter, K-wires were removed and cultured in tryptic soy broth and then 5% sheep blood agar mediums. Sliced sections were removed from the tibiae, stained with hematoxylin-eosin, and semi-quantitatively evaluated with X-rays. The addition of fosfomycin into PDL did not affect the X-ray and histopathological evaluation scores; however, the addition of gentamicin lowered them. The addition of gentamicin showed a protective effect after the 28th day of X-ray evaluations. PDL-only coating provided no protection, while adding fosfomycin to PDL offered a 20% level protection and adding gentamicin offered 80%. Furthermore, there were 103 CFU level growths in the gentamicin-added group, while the other groups had 105. Thus, the addition of fosfomycin to PDL does not affect the efficacy of prophylaxis, but the addition of gentamicin does. We therefore do not advise the use of fosfomycin as a single antimicrobial agent in coating for IRI prophylaxis.
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影响因子：
4.1
作者：
Lucke, M;Wildemann, B;Schmidmaier, G
通讯作者：
Schmidmaier, G
影响因子：
2.3
作者：
Fuchs T;Stange R;Schmidmaier G;Raschke MJ
通讯作者：
Raschke MJ
DOI：
10.1016/j.surge.2014.04.002
发表时间：
2015-10-01
影响因子：
2.5
作者：
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通讯作者：
Clift, B. A.
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3
作者：
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通讯作者：
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影响因子：
7.5
作者：
MEISSNER, A;HAAG, R;RAHMANZADEH, R
通讯作者：
RAHMANZADEH, R