Urinary biomarkers of exposure and of oxidative damage in children exposed to low airborne concentrations of benzene
Urinary biomarkers of exposure and of oxidative damage in children exposed to low airborne concentrations of benzene
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DOI：
10.1016/j.envres.2015.07.003
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发表时间：
2015-10-01
影响因子：
8.3
通讯作者：
Mutti, A.
中科院分区：
文献类型：
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作者：
Andreoli, R.;Spatari, G.;Mutti, A.
这项工作的目的是评估暴露于环境污染物和烟草烟雾(ETS)中的儿童(5-11岁)对核酸的氧化损伤。对每个受试者进行两次尿样(晚上和次日早上)，用串联LC-MS-MS测定8-氧代-7，8-二氢-2‘-脱氧鸟苷(8-oxodGuo)、8-oxo-7，8-二氢鸟苷(8-oxoguo)和8-oxo-7，8-二氢鸟苷(8-oxogua)等核酸氧化产物。甲基叔丁基醚(U-MTBE)、苯(U-BEZ)及其代谢产物(t，t-Muconic和S-苯硫基尿酸，t，t-MA和S-PMA)被确定为空气污染的生物标志物，可替宁作为ETS暴露的生物标志物。暴露的生物标志物(S-PMA和U-MTBE)和DNA氧化的生物标志物(8-oxodGuo)依赖于城市化和工业化水平，晚间样本与次日上午相比有所增加(p<；0.05)。在晚间和次日清晨，8-oxo和8-oxo与苯接触的生物标志物呈正相关(r=0.596和r=0.537，p&t；0.01)，特别是与S-PMA(r=0.59和r=0.45)，8-oxo分别为r=0.411和r=0.383。未观察到U-MTBE和可替宁的这种相关性。多元线性回归分析显示，8-oxodGuo与S-PMA在两个采样时间均呈正相关(晚上和次日上午分别为β=0.18和β=0.14；p
The aim of this work was to evaluate the oxidative damage to nucleic acids in children (5-11 years) associated with exposure to environmental pollutants and tobacco smoke (ETS). For each subject, urinary sampling was done twice (evening and next morning) to measure by tandem LC-MS-MS such oxidated products of nucleic acids as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and 8-oxo-7,8-dihydroguanine (8-oxoGua). Methyl tert-butyl ether (U-MTBE), benzene (U-Benz), and its metabolites (t,t-muconic and S-phenylmercapturic acids, t,t-MA and S-PMA, respectively) were determined as biomarkers of exposure to air pollution, and cotinine as a biomarker of exposure to ETS.Biomarkers of exposure (S-PMA and U-MTBE) and of DNA oxidation (8-oxodGuo) were dependent on the urbanization and industrialization levels and increased in the evening sample as compared to next morning (p < 0.05). In both evening and next morning samples, 8-oxodGuo and 8-oxoGuo correlated with each other (r=0.596 and r=0.537, respectively, p < 0.01) and with biomarkers of benzene exposure, particularly S-PMA (r=0.59 and r=0.45 for 8-oxodGuo and r=0.411 and r=0.383 for 8-oxoGuo, p < 0.01). No such correlations were observed for U-MTBE and cotinine. Multiple linear regression analyses showed that 8-oxodGuo was positively associated with S-PMA at both sampling times (beta=0.18 and beta=0.14 for evening and next morning sampling, respectively; p