Viscoelastic properties of fibrinogen adsorbed to the surface of biomaterials used in blood-contacting medical devices.
Viscoelastic properties of fibrinogen adsorbed to the surface of biomaterials used in blood-contacting medical devices.
复制标题
DOI：
10.1021/la060500r
复制
发表时间：
2007-02
期刊：
影响因子：
--
通讯作者：
N. Weber;A. Pesnell;D. Bolikal;J. Zeltinger;J. Kohn
中科院分区：
文献类型：
--
作者：
N. Weber;A. Pesnell;D. Bolikal;J. Zeltinger;J. Kohn
高分子血管植入物的血液相容性部分取决于纤维蛋白原吸附到植入物表面的倾向。采用带耗散监测（QCM-D）的石英晶体微天平实时测量纤维蛋白原表面吸附。六种新的、可生物降解的酪氨酸衍生聚碳酸酯被用作测试表面。不锈钢、聚（l -乳酸）、聚（D， l -乳酸-羟基乙酸酯）和聚（对苯二甲酸乙酯）表面作为对照，并将测试表面与常用生物材料的表面进行比较。我们的研究解决了一个问题，即聚合物结构的系统变化在多大程度上可以用来优化x射线可见性，并提供可调的降解率，同时产生蛋白质排斥表面特性，从而最大限度地减少纤维蛋白原吸附。QCM-D显示纤维蛋白原层厚度（2至37 nm），吸附湿质量（0.2至4.3 μ g/cm2）和粘度（0.001至0.005 kg/ms）的表面依赖性变化。虽然我们没有发现表面空气-水接触角测量与纤维蛋白原吸附之间的整体相关性（R2 = 0.08），但我们的数据表明，在具有相同聚合物主链的聚合物亚群中逐渐增加聚乙二醇含量将导致纤维蛋白原吸附减少。在该聚合物亚群中，空气-水接触角的减少与纤维蛋白原吸附的减少之间存在很强的相关性（R2 = 0.95）。我们得出的结论是，在优化x射线可见性和降解率的同时，可以最大限度地减少纤维蛋白原对酪氨酸衍生聚碳酸酯的吸附。一些酪氨酸衍生的聚碳酸酯被认为是设计血液接触植入物的有用材料，因为它们的纤维蛋白原吸附水平相对于常用的对照物要低得多。
The hemocompatibility of polymeric vascular implants is in part dependent on the propensity of fibrinogen to adsorb to the implant surface. Fibrinogen surface adsorption was measured in real time using a quartz crystal microbalance with dissipation monitoring (QCM-D). Six new, biodegradable tyrosine-derived polycarbonates were used as test surfaces. Stainless steel, poly(L-lactic acid), poly(D,L-lactide-co-glycolide), and poly(ethylene terephthalate) surfaces served as controls and provided a comparison of the test surfaces with those of commonly used biomaterials. Our study addressed the question regarding to which extent systematic variations in polymer structure can be used to optimize X-ray visibility and provide tunable degradation rates while generating protein-repellant surface properties that minimize fibrinogen adsorption. QCM-D revealed surface-dependent changes in fibrinogen layer thickness (2 to 37 nm), adsorbed wet mass (0.2 to 4.3 microg/cm2), and viscosity (0.001 to 0.005 kg/ms). While we did not find an overall correlation between surface air-water contact angle measurements and fibrinogen adsorption (R2 = 0.08), our data demonstrate that gradually increasing the poly(ethylene glycol) content within a subgroup of polymers having the same polymer backbone will lead to decreased fibrinogen adsorption. Within this subgroup of polymers, there was a strong correlation between decreasing air-water contact angles and decreasing fibrinogen adsorption (R2 = 0.95). We conclude that it is possible to minimize fibrinogen adsorption to tyrosine-derived polycarbonates while optimizing X-ray visibility and degradation rates. Some of the tyrosine-derived polycarbonates were identified as useful materials for the design of blood-contacting implants on the basis of their substantially lower levels of fibrinogen adsorption relative to the commonly used controls.