The number of platinum atoms binding to DNA, RNA and protein molecules of HeLa cells treated with cisplatin at its mean lethal concentration.
The number of platinum atoms binding to DNA, RNA and protein molecules of HeLa cells treated with cisplatin at its mean lethal concentration.
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DOI：
10.1111/j.1349-7006.1992.tb01959.x
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发表时间：
1992-05
期刊：
影响因子：
--
通讯作者：
Miyahara T
中科院分区：
文献类型：
--
作者：
Akaboshi M;Kawai K;Maki H;Akuta K;Ujeno Y;Miyahara T
用~(195)mPT标记的顺式氯铂(CDDP)在不同条件下处理HeLa-S-3细胞，研究其对HeLa-HeLa-S-3细胞的杀伤作用与铂原子结合数的关系。37℃处理细胞1、2、3h的平均致死浓度分别为2.8、2.0和1.1μg/ml。使用相同处理的细胞，用Schneider方法测定了细胞分离后与DNA、RNA和蛋白质分子结合的铂原子的数量。用药物浓度表示的DO值代替与各组分结合的铂原子个数，计算出各组分DO值的倒数表示的靶区体积。结果表明，DNA是CDDP杀伤细胞的主要靶点，其靶向体积分别为5.17×104、5.71×104和1.03×104个核苷酸。
HeLa S‐3 cells were treated with 195mPt‐radiolabeled cis‐diamminedichloroplatinum(II) (CDDP) under various conditions, and the relationship between lethal effect and the number of Pt atoms binding to DNA, RNA and proteins was examined. The mean lethal concentrations for the cells treated with CDDP at 37°C for 1, 2 and 3 h were 2.8, 2.0 and 1.1 μg/ml, respectively. By using identically treated cells, the number of Pt atoms combined with DNA, RNA and protein molecules were determined after fractionation of the cells using the method of Schneider. In this way, the Do values given as the drug concentration were substituted for the number of Pt atoms combined with each fraction, then the target volumes expressed as the reciprocals of Do values were calculated for each fraction. The results provide strong support for the idea that DNA is the primary target for cell killing by CDDP, and the target volumes were 5.17 × 104, 5.71 × 104 and 1.03 × 104 nucleotides for 1, 2 and 3 h treated cells, respectively.