Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy
Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy
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DOI：
10.1117/1.3374049
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发表时间：
2010-03-01
影响因子：
3.5
通讯作者：
Marcu, Laura
中科院分区：
文献类型：
--
作者：
Butte, Pramod V.;Fang, Qiyin;Marcu, Laura
本研究的目的是确定时间分辨激光诱导荧光光谱（TR-LIFS）作为脑肿瘤与周围正常组织描绘的辅助工具的潜力，以协助神经外科医生进行近乎完全的肿瘤切除。使用时域TR-LIFS原型仪（门控光电倍增管检测，快速数字化仪）记录术中正常皮层（NC）、正常白质（NWM）和不同级别胶质瘤的组织自身荧光。用脉冲氮激光（337 nm, 700 ps）诱导组织荧光，在360 ~ 550 nm光谱范围（10 nm间隔）记录荧光强度衰减曲线。组织病理学分析（苏木精和伊红）从TR-LIFS测量部位采集的活检样本用于光谱结果的验证。17例患者的初步结果显示，正常皮质（N=16）和正常白质（N=3）在390 nm（寿命=1.8 +/- 0.3 ns）和460 nm（寿命=0.8 +/- 0.1 ns）处出现两个荧光发射峰。在低级别胶质瘤（N=5，寿命=1.1 ns）中没有390-nm发射峰，在高级别胶质瘤（N=9，寿命=1.7±0.4 ns）中减弱。各组织460 nm处的发射特性与烟酰胺腺嘌呤二核苷酸荧光相关（峰值440 ~ 460 nm，寿命0.8 ~ 1.0 ns）。这些发现证明了在手术中使用TR-LIFS作为增强描述脑肿瘤的工具的潜力。此外，本研究还评估了在体内获得的脑肿瘤的TR-LIFS特征与先前报道的在体外脑肿瘤标本中的特征之间的异同。(C) 2010光学仪器工程师学会。(DOI: 10.1117/1.3374049)
The goal of this study is to determine the potential of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) as an adjunctive tool for delineation of brain tumor from surrounding normal tissue in order to assist the neurosurgeon in near-complete tumor excision. A time-domain TR-LIFS prototype apparatus (gated photomultiplier detection, fast digitizer) was used for recording tissue autofluorescence in normal cortex (NC), normal white matter (NWM), and various grades of gliomas intraoperatively. Tissue fluorescence was induced with a pulsed nitrogen laser (337 nm, 700 ps), and the intensity decay profiles were recorded in the 360- to 550-nm spectral range (10-nm interval). Histopathological analysis (hematoxylin & eosin) of the biopsy samples taken from the site of TR-LIFS measurements was used for validation of spectroscopic results. Preliminary results on 17 patients demonstrate that normal cortex (N=16) and normal white matter (N=3) show two peaks of fluorescence emission at 390 nm (lifetime=1.8 +/- 0.3 ns) and 460 nm (lifetime=0.8 +/- 0.1 ns). The 390-nm emission peak is absent in low-grade glioma (N=5; lifetime=1.1 ns) and reduced in high-grade glioma (N=9; lifetime=1.7 +/- 0.4 ns). The emission characteristics at 460 nm in all tissues correlated with the nicotinamide adenine dinucleotide fluorescence (peak: 440 to 460 nm; lifetime: 0.8 to 1.0 ns). These findings demonstrate the potential of using TR-LIFS as a tool for enhanced delineation of brain tumors during surgery. In addition, this study evaluates similarities and differences between TR-LIFS signatures of brain tumors obtained in vivo and those previously reported in ex vivo brain tumor specimens. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3374049]