Effects of tamoxifen on human breast cancer cell cycle kinetics: accumulation of cells in early G1 phase.
Effects of tamoxifen on human breast cancer cell cycle kinetics: accumulation of cells in early G1 phase.
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DOI：
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发表时间：
1983-08
期刊：
影响因子：
11.2
通讯作者：
C. Osborne;C. Osborne;D. Boldt;G. Clark;J. Trent
中科院分区：
文献类型：
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作者：
C. Osborne;C. Osborne;D. Boldt;G. Clark;J. Trent
我们研究了他莫昔芬对内分泌反应性MCF-7人乳腺癌细胞周期动力学的影响。他莫昔芬抑制MCF-7细胞增殖。他莫昔芬显着降低了氚化胸苷标记指数，表明S期细胞比例减少。光红霉素染色的细胞的流式细胞术显示，细胞在G1期积累，伴随着S-和G2-M-期细胞与他莫昔芬耗尽。通过过早凝聚的染色体形态学对G1期细胞进行的绘图表明，他莫昔芬处理的细胞在G1早期积累。这些研究表明，他莫昔芬通过在细胞周期的G1期早期引起过渡延迟来抑制MCF-7人乳腺癌细胞的增殖。
We have studied the effects of tamoxifen on the cell cycle kinetics of the endocrine-responsive MCF-7 human breast cancer cells. Tamoxifen inhibits proliferation of MCF-7 cells. The tritiated thymidine labeling index is markedly reduced by tamoxifen, indicating a reduction in the fraction of cells in S phase. Flow cytometry of mithramycin-stained cells reveals that cells accumulate in G1 phase, with a concomitant depletion of S- and G2-M-phase cells with tamoxifen. Mapping of G1-phase cells by morphology of prematurely condensed chromosomes demonstrated that tamoxifen-treated cells accumulate in early G1. These studies indicate that tamoxifen inhibits proliferation of MCF-7 human breast cancer cells by invoking a transition delay early in the G1 phase of the cell cycle.