Zebrafish mast cells possess an FcεRI-like receptor and participate in innate and adaptive immune responses
Zebrafish mast cells possess an FcεRI-like receptor and participate in innate and adaptive immune responses
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DOI：
10.1016/j.dci.2010.09.001
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发表时间：
2011-01-01
影响因子：
2.9
通讯作者：
Berman, Jason N.
中科院分区：
文献类型：
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作者：
Da'as, Sahar;Teh, Evelyn M.;Berman, Jason N.
我们之前鉴定了斑马鱼肥大细胞（MC）谱系，现在的目标是确定这些细胞是否在先天和适应性免疫中类似于它们的哺乳动物对应物。腹腔注射复方48/80或活的沙门氏菌气单胞菌，与注射盐水的对照组相比，在组织学上和血浆胰蛋白酶水平上均有显著的MC脱粒（p分别为0.0006和0.005）。酮替芬预处理消除了这些反应（p分别= 0.0004,0.005）。在斑马鱼体内观察到抗人高亲和力IgE受体γ (FceRI γ)和IgE重链抗体的交叉反应性。7天胚胎的全坐骨原位杂交表明，mc特异性标记cpa5与myd88 （toll样受体接头）和斑马鱼Fc epsilon RI亚基同源物共定位。与不匹配的对照组相比，注射了二硝基苯致敏小鼠（抗dnp） IgE和DNP-BSA或三硝基苯致敏小鼠（抗tnp） IgE和TNP-BSA的IP的斑马鱼血浆胰蛋白酶增加（p分别为0.03和0.010）。这些结果证实了功能守恒，并验证了斑马鱼模型作为新型MC调节剂的体内筛选工具。(C) 2010 Elsevier Ltd.版权所有。
We previously identified a zebrafish mast cell (MC) lineage and now aim to determine if these cells function analogously in innate and adaptive immunity like their mammalian counterparts. Intraperitoneal (IP) injection of compound 48/80 or live Aeromonas salmonicida resulted in significant MC degranulation evident histologically and by increased plasma tryptase compared with saline-injected controls (p = 0.0006, 0.005, respectively). Pre-treatment with ketotifen abrogated these responses (p = 0.0004, 0.005, respectively). Cross-reactivity was observed in zebrafish to anti-human high-affinity IgE receptor gamma (FceRI gamma) and IgE heavy chain-directed antibodies. Whole mount in situ hybridization on 7-day embryos demonstrated co-localization of cpa5, a MC-specific marker, with myd88, a toll-like receptor adaptor, and zebrafish Fc epsilon RI subunit homologs. Zebrafish injected IP with matched dinitrophenyl-sensitized mouse (anti-DNP) IgE and DNP-BSA or trinitrophenyl-sensitized mouse (anti-TNP) IgE and TNP-BSA demonstrated increased plasma tryptase compared with mismatched controls (p = 0.03, 0.010, respectively). These results confirm functional conservation and validate the zebrafish model as an in vivo screening tool for novel MC modulating agents. (C) 2010 Elsevier Ltd. All rights reserved.