The Maize Leaf Starch Mutation agps-m1 Has Diminished Field Growth and Productivity
The Maize Leaf Starch Mutation agps-m1 Has Diminished Field Growth and Productivity
复制标题
DOI：
10.2135/cropsci2011.03.0158
复制
发表时间：
2012-03-01
期刊：
影响因子：
2.3
通讯作者：
Giroux, Michael J.
中科院分区：
文献类型：
--
作者：
Schlosser, Alanna J.;Martin, John M.;Giroux, Michael J.
改变与库或源强度有关的酶是提高谷物产量的一种常用方法。ADP-葡萄糖焦磷酸化酶（ADP-glucose pyrophosphorylase，AGP-glucose pyrophosphorylase，AGP-glucose pyrophosphorylase）是植物叶片和种子淀粉合成的限速酶，因此受到广泛关注。以前的研究，重点是增加种子中的AGEs活性，表明只有当环境资源不受限制时，生产力才会增加。本研究的目的是确定叶淀粉对玉米生产力和生长的重要性。对agps-m1突变体和叶片淀粉分离的BC 4F 2：3植株进行田间试验。结果清楚地表明正常叶片淀粉水平对玉米生产力的重要性。无淀粉agps-m1植株比其野生型姊妹株矮6 - 15 cm，开花晚2 - 6 d，产量低30%。野生型和agps-m1植株的光合速率没有显著差异。然而，agps-m1叶的主要光合蛋白，如磷酸烯醇丙酮酸羧化酶，丙酮酸磷酸二激酶，RuBisCO的水平显着降低，直到光周期结束。结果表明，在农艺条件下，叶淀粉和整株植物生产力之间存在联系。进一步的研究将有助于确定叶片淀粉如何调节叶片光合蛋白的产生。
Modification of enzymes involved in sink or source strength has been one common method used in attempts to improve cereal yield. In this regard, ADP-glucose pyrophosphorylase (AGPase) has received considerable attention because it controls the rate-limiting step in starch biosynthesis in both leaves and seeds. Previous studies, focused on increasing AGPase activity in seeds, indicate that productivity increases occur only when environmental resources are nonlimiting. The objective of this study was to determine the importance of leaf starch on the productivity and growth of maize. Field trials of BC4F2:3 plants segregating for the agps-m1 mutation and leaf starch were conducted. The results clearly demonstrate the importance of normal leaf starch levels to maize productivity. The starchless agps-m1 plants were 6 to 15 cm shorter, flowered 2 to 6 d later, and were 30% lower yielding than their wild-type sister lines. Photosynthetic rates were not significantly different between wild-type and agps-m1 plants. However, agps-m1 leaves were significantly lower in the levels of major photosynthetic proteins such as phosphoenolpyruvate carboxylase, pyruvate phosphate dikinase, and RuBisCO until the end of the photoperiod. The results demonstrate a link between leaf starch and whole plant productivity under agronomic conditions. Further study would help to define how leaf starch regulates the production of leaf photosynthetic proteins.