Interleukin-1 Activates an NF-B-like Factor in Osteoclast-like Cells (*)
Interleukin-1 Activates an NF-B-like Factor in Osteoclast-like Cells (*)
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Interleukin-1 激活破骨细胞样细胞中的 NF-B 样因子 (*)
DOI：
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发表时间：
1996
影响因子：
4.8
通讯作者：
T. Koga
中科院分区：
文献类型：
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作者：
E. Jimi;T. Ikebe;N. Takahashi;M. Hirata;T. Suda;T. Koga
我们研究了破骨细胞样细胞中的 NF-κB 转录因子。在胶原凝胶包被的培养皿中，在 1α,25-二羟基维生素 D 和前列腺素 E 存在的情况下，与小鼠颅骨来源的原代成骨细胞共培养，将破骨细胞样细胞从小鼠骨髓细胞中分化出来。我们通过链霉蛋白酶处理从共培养物中富集了破骨细胞样细胞。当用佛波醇 12-肉豆蔻酸酯 13-乙酸酯、白介素-1 (IL-1)、降钙素或巨噬细胞集落刺激因子处理富集的破骨细胞样细胞时，只有 IL-1 激活 NF-κB 样因子，该因子特异性结合 κB 基序 DNA 序列，通过电泳迁移率变动测定检测到。 IL-1 还激活成骨细胞中的 NF-κB 诱导。然而，电泳迁移率变动分析显示，由 IL-1 刺激的破骨细胞样细胞诱导的 NF-κB 样因子的分子大小比成骨细胞中的因子更小。破骨细胞样细胞的 NF-κB 活性完全被抗 p50 亚基的抗体识别，而仅部分被抗 NF-κB p65 亚基的抗体识别。针对 c-Rel、Rel B 和 p52 的抗体不能识别 NF-κB 样因子。这些结果表明，IL-1 激活破骨细胞样细胞中的 NF-κB 样因子，该细胞含有 p50 和 p65 相关蛋白。
We investigated the NF-κB transcription factor in osteoclast-like cells. Osteoclast-like cells were differentiated from mouse bone marrow cells in co-culture with mouse calvaria-derived primary osteoblasts in the presence of 1α,25-dihydroxyvitamin D and prostaglandin E in collagen gel-coated dishes. We enriched osteoclast-like cells from the co-cultures by Pronase treatment. When the enriched osteoclast-like cells were treated with phorbol 12-myristate 13-acetate, interleukin-1 (IL-1), calcitonin, or macrophage colony-stimulating factor, only IL-1 activated an NF-κB-like factor, which specifically bound to a κB motif DNA sequence, as detected by an electrophoretic mobility shift assay. IL-1 also activated NF-κB induction in osteoblasts. However, the NF-κB-like factor induced by IL-1-stimulated osteoclast-like cells is of smaller molecular size than the factor in osteoblasts, as shown by an electrophoretic mobility shift assay. The NF-κB activity of osteoclast-like cells was recognized completely by antibodies against the p50 subunit, and only partially by antibodies against the p65 subunit of NF-κB. Antibodies against c-Rel, Rel B, and p52 did not recognize the NF-κB-like factor. These results suggest that IL-1 activates an NF-κB-like factor in osteoclast-like cells, which contains p50 and p65-related proteins.