A STUDY OF CALCIFICATION IN THE LEG TENDONS FROM THE DOMESTIC TURKEY
A STUDY OF CALCIFICATION IN THE LEG TENDONS FROM THE DOMESTIC TURKEY
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DOI：
10.1016/0889-1605(86)90069-8
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发表时间：
1986-03-01
期刊：
影响因子：
--
通讯作者：
LANDIS, WJ
中科院分区：
文献类型：
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作者：
LANDIS, WJ
详细介绍了从国内火鸡，Meleagris gallopavo腿肌腱内的钙化的结构和空间和时间顺序。组织的组织学和超微结构检查揭示了大tendonoblasts的发展，设置在列平行于肌腱长轴。成熟细胞的特征是细胞器在蛋白质合成中活跃，线粒体颗粒很少，过程系统广泛。矿物质在细胞外组织间隙中的沉积最初似乎与位于胶原纤维之间的小泡状结构相关。随着钙化的进展，矿物质主要与胶原蛋白相关。电子衍射和电子探针显微分析表明，该矿物是一种结晶度差的羟基磷灰石，Ca/P摩尔比在0.9和1.5之间。在组织切片脱钙和染色后，囊泡结构通过吸收铅和铀盐保持完整性，而胶原蛋白染色较差。这些结果的影响进行了讨论相对于脊椎动物钙化的物理化学和生物学事件。
Details are presented of the structure and the spatial and temporal sequence of calcification within leg tendons obtained from the domestic turkey, Meleagris gallopavo. Histological and ultrastructural examination of the tissues reveals the development of large tendonoblasts, disposed in columns parallel to tendon long axes. Maturing cells are characterized by organelles active in protein synthesis, few mitochondrial granules and an extensive system of processes. Deposition of mineral in the extracellular tissue spaces appears initially associated with small vesicular structures located between collagen fibrils. As calcification progresses, mineral is associated predominantly with collagen. Electron diffraction and electron probe microanalysis indicate that the mineral is a poorly crystalline hydroxyapatite having a Ca/P molar ratio ranging between 0.9 and 1.5. Following decalcification and staining of tissue sections, vesicular structures retain integrity by uptake of lead and uranium salts while collagen stains only poorly. Implications of these results are discussed with respect to the physicochemical and biological events of vertebrate calcification.