Nitrate and ammonium lead to distinct global dynamic phosphorylation patterns when resupplied to nitrogen-starved Arabidopsis seedlings.
Nitrate and ammonium lead to distinct global dynamic phosphorylation patterns when resupplied to nitrogen-starved Arabidopsis seedlings.
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DOI：
10.1111/j.1365-313x.2011.04848.x
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发表时间：
2012-03
期刊：
影响因子：
--
通讯作者：
Schulze WX
中科院分区：
文献类型：
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作者：
Engelsberger WR;Schulze WX
氮素是植物生长发育所必需的大量营养元素。无机氮及其同化产物控制着各种代谢、生理和发育过程。虽然氮诱导的转录反应已被广泛研究，在过去，我们的工作集中在发现候选蛋白质的调控事件是互补的转录变化。大多数信号传导途径涉及通过蛋白磷酸化调节蛋白丰度和/或活性。因此，我们分析了蛋白质磷酸化的动态变化，在膜和可溶性蛋白质暴露于快速变化的养分供应超过30分钟的时间过程中的植物。植物饥饿的氮，随后再供应氮的形式，硝酸盐或铵。在氮再供应后5分钟内磷酸化水平变化最大的蛋白质（快速反应）包括GPI锚定蛋白、受体激酶和转录因子，而在氮再供应10分钟后磷酸化水平变化最大的蛋白质（晚期反应）包括参与蛋白质合成和降解的蛋白质，以及具有中枢代谢和激素代谢功能的蛋白质。以硝酸盐或铵的形式再供应氮导致不同的磷酸化模式，主要是具有信号功能的蛋白质、转录因子和转运蛋白。
Nitrogen is an essential macronutrient for plant growth and development. Inorganic nitrogen and its assimilation products control various metabolic, physiological and developmental processes. Although the transcriptional responses induced by nitrogen have been extensively studied in the past, our work here focused on the discovery of candidate proteins for regulatory events that are complementary to transcriptional changes. Most signaling pathways involve modulation of protein abundance and/or activity by protein phosphorylation. Therefore, we analyzed the dynamic changes in protein phosphorylation in membrane and soluble proteins from plants exposed to rapid changes in nutrient availability over a time course of 30 min. Plants were starved of nitrogen and subsequently resupplied with nitrogen in the form of nitrate or ammonium. Proteins with maximum change in their phosphorylation level at up to 5 min after nitrogen resupply (fast responses) included GPI-anchored proteins, receptor kinases and transcription factors, while proteins with maximum change in their phosphorylation level after 10 min of nitrogen resupply (late responses) included proteins involved in protein synthesis and degradation, as well as proteins with functions in central metabolism and hormone metabolism. Resupply of nitrogen in the form of nitrate or ammonium resulted in distinct phosphorylation patterns, mainly of proteins with signaling functions, transcription factors and transporters.